Ruppert L D, Drackley J K, Bremmer D R, Clark J H
Department of Animal Sciences, University of Illinois, Urbana 61801, USA.
J Dairy Sci. 2003 Feb;86(2):593-609. doi: 10.3168/jds.S0022-0302(03)73638-8.
Six multiparous Holstein cows (average 31 days in milk; 36.3 kg/d of milk) fitted with ruminal cannulas were used in a 6 x 6 Latin square with 21-d periods to investigate the effects of diets that varied in forage source and amount of supplemental tallow. Isonitrogenous diets in a 2 x 3 factorial arrangement were based on either high corn silage (40:10 corn silage to alfalfa silage, % of dry matter) or high alfalfa silage (10:40 corn silage to alfalfa silage, % of dry matter) and contained 0, 2, or 4% tallow. Intakes of dry matter and total fatty acids were lower when cows were fed the high corn silage diet. Tallow supplementation linearly decreased dry matter intake. Milk yield was unaffected by diet; yields of milk fat and 3.5% fat-corrected milk were higher for the high alfalfa silage diet but were unaffected by tallow. Milk fat percentage was higher for the high alfalfa silage and tended to decrease when tallow was added to the high corn silage diet. Contents of trans-C18:1 isomers in milk fat were increased by high corn silage and tallow, and tended to be increased more when tallow was fed in the high corn silage diet. Ruminal pH and acetate:propionate were lower when high corn silage was fed. Ruminal acetate:propionate decreased linearly as tallow increased; the molar proportion of acetate was decreased more when tallow was added to the high corn silage diet. Ruminal liquid dilution rates were higher for the alfalfa silage diet; ruminal volume and solid passage rates were similar among diets. Total tract apparent digestibilities of dry matter, organic matter, crude protein, starch, energy, and total fatty acids were unaffected by diet. Digestibilities of neutral detergent fiber, acid detergent fiber, hemicellulose, and cellulose were lower when high corn silage was fed. The high alfalfa silage diet increased intakes of metabolizable energy and N, and increased milk energy and productive N. Tallow decreased the amount of N absorbed but had few other effects on utilization of energy or N. Tallow linearly increased concentrations of nonesterified fatty acids and cholesterol in plasma; cholesterol was increased by high alfalfa silage. Overall, forage source had more pronounced effects on production and metabolism than did tallow supplementation. Few interactions between forage source and tallow supplementation were detected except that ruminal fermentation and milk fat content were affected more negatively when tallow was fed in the high corn silage diet.
选用6头经产荷斯坦奶牛(平均产奶31天;日产奶量36.3千克),这些奶牛安装了瘤胃瘘管,采用6×6拉丁方设计,试验期为21天,以研究不同饲草来源和补充牛脂量的日粮的影响。采用2×3析因设计的等氮日粮,分别以高玉米青贮料(玉米青贮料与苜蓿青贮料比例为40:10,干物质百分比)或高苜蓿青贮料(玉米青贮料与苜蓿青贮料比例为10:40,干物质百分比)为基础,并含有0%、2%或4%的牛脂。给奶牛饲喂高玉米青贮料日粮时,干物质和总脂肪酸摄入量较低。补充牛脂使干物质摄入量呈线性下降。日粮对产奶量无影响;高苜蓿青贮料日粮的乳脂产量和3.5%乳脂校正乳产量较高,但不受牛脂影响。高苜蓿青贮料日粮的乳脂率较高,而在高玉米青贮料日粮中添加牛脂时乳脂率有下降趋势。高玉米青贮料和牛脂使乳脂中反式C18:1异构体含量增加,在高玉米青贮料日粮中添加牛脂时增加趋势更明显。饲喂高玉米青贮料时瘤胃pH值和乙酸:丙酸比值较低。随着牛脂添加量增加,瘤胃乙酸:丙酸比值呈线性下降;在高玉米青贮料日粮中添加牛脂时乙酸的摩尔比例下降幅度更大。苜蓿青贮料日粮的瘤胃液稀释率较高;日粮间瘤胃容积和固体通过率相似。日粮对干物质、有机物、粗蛋白、淀粉、能量和总脂肪酸的全肠道表观消化率无影响。饲喂高玉米青贮料时,中性洗涤纤维、酸性洗涤纤维、半纤维素和纤维素的消化率较低。高苜蓿青贮料日粮增加了可代谢能量和氮的摄入量,提高了乳能量和生产性氮。牛脂减少了氮的吸收量,但对能量或氮的利用影响较小。牛脂使血浆中非酯化脂肪酸和胆固醇浓度呈线性增加;高苜蓿青贮料使胆固醇增加。总体而言,饲草来源对生产和代谢的影响比补充牛脂更为显著。除了在高玉米青贮料日粮中添加牛脂时瘤胃发酵和乳脂含量受到更负面的影响外,未检测到饲草来源和补充牛脂之间的显著相互作用。