粪肠球菌蛋白EF0377的纯化、结晶及X射线初步分析。
Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.
作者信息
Riboldi-Tunnicliffe Alan, Bent Colin J, Isaacs Neil W, Mitchell Timothy J
机构信息
IBLS Infection and Immunity, University of Glasgow, Scotland.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):755-7. doi: 10.1107/s0907444903003718. Epub 2003 Mar 25.
The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.
粪肠球菌的EF0377基因被克隆并在大肠杆菌中过表达。该蛋白质已被纯化并形成三种晶体形式。III型晶体属于空间群P2(1)，晶胞参数a = 72.11，b = 94.97，c = 80.77 Å，β = 111.93°。每个不对称单元有四个分子，在低温条件下（100 K）使用同步辐射观察到衍射极限达到1.65 Å。从天然晶体收集了一套几乎完整的X射线衍射数据，分辨率达到1.9 Å。
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