CD34+细胞转导外源性Fas配体诱导白血病细胞凋亡。

Apoptosis of leukemia cells induced by CD34+ cells transferred exogenous Fas ligand.

作者信息

Xiao Juan, Zou Ping, Liu Zhongwen, Hu Zhongbo, Liu Lingbo

机构信息

Institute of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2002;22(3):197-9. doi: 10.1007/BF02828178.

DOI:10.1007/BF02828178

PMID:12658802

Abstract

To assess the value of CD34+ cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34+ cells transfected with FasL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara-C). After 18 h, apoptosis of cells was detected by FCM and TUNEL. Induced for 18 h by CD34+ cells transfected with FasL or without, the ratio of apoptosis of U937 cells was (5.0 +/- 1.3)%, (10.8 +/- 0.6)% (P < 0.01), respectively. Induced by FasL+ CD34+ + DNR, FasL+ CD34+ + Ara-C, the ratio was (13.4 +/- 1.0)% (P < 0.05), (17.9 +/- 1.3)% (P < 0.01), respectively. The result demonstrated that CD34+ cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.

摘要

为评估转染外源性Fas配体（FasL）的CD34+细胞在诱导人白血病细胞凋亡中的价值，将转染或未转染FasL且经丝裂霉素C预处理的CD34+细胞，在有或无柔红霉素（DNR）或阿糖胞苷（Ara-C）存在的情况下，与白血病细胞系U937细胞混合。18小时后，通过流式细胞术（FCM）和末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测细胞凋亡情况。转染或未转染FasL的CD34+细胞诱导18小时后，U937细胞的凋亡率分别为（5.0±1.3）%、（10.8±0.6）%（P<0.01）。FasL+CD34++DNR、FasL+CD34++Ara-C诱导后，凋亡率分别为（13.4±1.0）%（P<0.05）、（17.9±1.3）%（P<0.01）。结果表明，转染外源性FasL的CD34+细胞可诱导人白血病细胞凋亡，且与化疗药物联合使用时显示出细胞毒性协同效应，提示有可能开发出一种治疗白血病的新方法。