阴道毛滴虫：在一种囊泡状亚细胞组分中鉴定出一种依赖磷脂酶A的溶血活性。

Trichomonas vaginalis: identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction.

作者信息

Vargas-Villarreal Javier, Mata-Cárdenas Benito D, González-Salazar Francisco, Lozano-Garza Hector G, Cortes-Gutierrez Elva I, Palaclos-Corona Rebeca, Martínez-Rodríguez Herminia G, Ramírez-Bon Enrique, Said-Fernández Salvador

机构信息

División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Administración de Correos No. 4, Apartado Postal 020-E, Colonia Independencia, Monterrey, CP 64720, Nuevo León, Mexico.

出版信息

J Parasitol. 2003 Feb;89(1):105-12. doi: 10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2.

DOI:10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2

PMID:12659311

Abstract

Trichomonad total extracts (TTE), or vesicular (P30) and soluble (530) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3. GT-13. and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0. in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 16.71 +/- 0.33 hemolytic units IHU]/mg/hr to 11.60 +/- 0.24 HU/mg/hr) than at pH 8.0 (3.81 +/- 0.30 HU/mg/hr to 5.75 +/- 0.65 HU/mg/hr). and it was greater than that on human red blood cells at pH 6.0 (2.67 +/- 0.19 HU/mg/hr to 4.08 +/- 0.15 HU/mg/hr) or pH 8.0 (2.24 +/- 0.0 9 HU/mg/hr to 2.81 +/- 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 microM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.

摘要

来自3株致病性阴道毛滴虫菌株（GT-3、GT-13和GT-15）的滴虫总提取物（TTE），以及囊泡（P30）和可溶性（530）亚细胞组分，以时间和剂量依赖性方式裂解人和斯普拉格-道利大鼠的红细胞。TTE的全部溶血活性位于P30中，在1 mM Ca2+存在下，显示出两个最大活性峰，一个在pH 6.0，另一个在pH 8.0。在pH 6.0时对大鼠红细胞的溶血活性（16.71±0.33溶血单位[HU]/mg/hr至11.60±0.24 HU/mg/hr）高于pH 8.0时（3.81±0.30 HU/mg/hr至5.75±0.65 HU/mg/hr），并且在pH 6.0（2.67±0.19 HU/mg/hr至4.08±0.15 HU/mg/hr）或pH 8.0（2.24±0.09 HU/mg/hr至2.81±0.06 HU/mg/hr）时对人红细胞的溶血活性也更高。80 microM罗森塔尔抑制剂的作用使碱性和酸性溶血活性降低（pH 6.0时降低60-93%，pH 8.0时降低78-93%），该抑制剂还分别抑制滴虫碱性和酸性磷脂酶A活性的27-45%和29-54%。囊泡、液泡和氢化酶体富含P30。阴道毛滴虫具有一种溶血磷脂酶A，这可能与其细胞致病机制有关。