Noguchi Shunsuke, Sone Nobuhito, Kawamura Masaru
Department of Biochemical Engineering and Science, Kyushu Institute of Technology Iizuka, Fukuoka 820-8502, Japan.
J Cell Sci. 2003 May 15;116(Pt 10):1875-80. doi: 10.1242/jcs.00392. Epub 2003 Mar 18.
We examined the effect of the beta-subunits of the Na+/K+ and H+/K+ ATPases on the biogenesis of the sarcoplasmic reticulum (SR) Ca2+ ATPase in Xenopus oocytes. Oocytes were simultaneously injected with cRNAs for both the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ or the H+/K+ ATPase. Immunoprecipitation with antiserum specific for the beta-subunit of the Na+/K+ or the H+/K+ ATPase yielded not only the respective beta-subunit but also the SR Ca2+ ATPase, indicating that the SR Ca2+ ATPase was associated with the beta-subunits of the Na+/K+ and the H+/K+ ATPases. Pulse-chase experiments revealed that the complex between the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ ATPase was formed transiently and dissociated during the course of maturation. This is the first report that demonstrates the association of the SR Ca2+ ATPase with the beta-subunit of the Na+/K+ and H+/K+ ATPases.
我们研究了Na⁺/K⁺ATP酶和H⁺/K⁺ATP酶的β亚基对非洲爪蟾卵母细胞肌浆网（SR）Ca²⁺ATP酶生物合成的影响。卵母细胞同时被注射了SR Ca²⁺ATP酶以及Na⁺/K⁺或H⁺/K⁺ATP酶β亚基的编码RNA（cRNAs）。用对Na⁺/K⁺或H⁺/K⁺ATP酶β亚基具有特异性的抗血清进行免疫沉淀，不仅得到了各自的β亚基，还得到了SR Ca²⁺ATP酶，这表明SR Ca²⁺ATP酶与Na⁺/K⁺和H⁺/K⁺ATP酶的β亚基相关联。脉冲追踪实验显示，SR Ca²⁺ATP酶与Na⁺/K⁺ATP酶β亚基之间的复合物是短暂形成的，并在成熟过程中解离。这是首次证明SR Ca²⁺ATP酶与Na⁺/K⁺和H⁺/K⁺ATP酶β亚基存在关联的报告。
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