皂荚果实提取物对人实体瘤细胞系的抗增殖活性。
Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines.
作者信息
Chow L M C, Tang J C O, Teo I T N, Chui C H, Lau F Y, Leung T W T, Cheng G, Wong R S M, Wong I L K, Tsang K M S, Tan W Q, Zhao Y Z, Lai K B, Lam W H, Guo D A, Chan A S C
机构信息
Department of Applied Biology and Chemical Technology and Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, Hong Kong Polytechnic University, China.
出版信息
Chemotherapy. 2002;48(6):303-8. doi: 10.1159/000069713.
BACKGROUND
The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponin-rich. However, its activity on solid tumour cell lines has never been demonstrated.
METHODS
The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection.
RESULTS
The mean MTT(50) (the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 microg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent.
CONCLUSIONS
Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.
背景
皂荚果实提取物（GSE）是一种富含皂苷的传统草药。然而，其对实体瘤细胞系的活性尚未得到证实。
方法
采用MTT法、非锚定依赖性克隆形成试验、DNA梯状条带分析和原位细胞死亡检测，在四种癌细胞系（乳腺癌MCF-7、MDA-MB231、肝母细胞瘤HepG2和食管鳞状癌细胞系SLMT-1）中证实GSE的活性。
结果
MTT（50）（使MTT活性降低50%的GSE平均浓度）范围为16至20微克/毫升GSE。非锚定依赖性克隆形成试验表明，用GSE处理后，所有四种实体瘤细胞系逐渐失去其再生潜力，DNA片段化和TUNEL分析表明GSE的作用具有剂量和时间依赖性。
结论
我们的结果表明，GSE具有细胞毒性活性，可诱导人实体瘤细胞系凋亡。
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