Wang Gangshi, Wang Mengwei, Wu Benyan, You Weidi
Department of Gastroenterology, General Hospital of Chinese PLA. Beijing, 100853 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Apr;20(2):119-22.
To clone human gastric cancer related gene and to analyze its expression profile in gastric mucosal tissues.
Paired tumor, paratumor and non-tumor specimens from 7 gastric adenocarcinoma patients (male 4, female 3, with average age 51 +/- 18 years) were studied by means of fluorescent differential display reverse transcription polymerase chain reaction (DDRT-PCR). The differentially expressed cDNA bands of interest were cloned and analyzed by Northern blot and in situ hybridization. Thirty cases (male 23 female 7 with average age 59 +/- 8 years) of paired paraffin-embedded gastric tumor and non-tumor tissues were used in in situ hybridization analysis.
A gene expressed much lower in 6 out of 7 tested tumor samples than in their normal and paratumor counterparts was identified. It was named GCRG123. Northern blot analysis confirmed the differential expression. Human multiple tissue Northern blot analysis showed that GCRG123 expressed in various adult human tissues including thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Sequence analysis revealed that GCRG123 (GenBank accession number AF454554) was a lamin like protein gene. It had one open reading frame which consisted of 49 amino acids (GenBank accession number AAL61668.1). In situ hybridization analysis showed a high GCRG123 expression level in normal gastric epithelium and pylori glands, but low expression level in tumor as well as dysplasia and most intestinal metaplasia at the paratumor regions.
A lamin-like protein gene was identified in human gastric mucosa, it is down-regulated in gastric cancer and its precancerous leisions.
克隆人胃癌相关基因并分析其在胃黏膜组织中的表达谱。
采用荧光差异显示逆转录聚合酶链反应（DDRT-PCR）对7例胃腺癌患者（男4例，女3例，平均年龄51±18岁）的肿瘤、瘤旁和非肿瘤配对标本进行研究。对感兴趣的差异表达cDNA条带进行克隆，并通过Northern印迹和原位杂交分析。30例（男23例，女7例，平均年龄59±8岁）配对的石蜡包埋胃肿瘤和非肿瘤组织用于原位杂交分析。
在7个检测的肿瘤样本中的6个中，发现一个基因的表达明显低于其正常和瘤旁对应组织。将其命名为GCRG123。Northern印迹分析证实了差异表达。人多组织Northern印迹分析表明，GCRG123在包括胸腺、前列腺、睾丸、卵巢、小肠、结肠和外周血白细胞在内的多种成人组织中表达。序列分析显示，GCRG123（GenBank登录号AF454554）是一个核纤层样蛋白基因。它有一个由49个氨基酸组成的开放阅读框（GenBank登录号AAL61668.1）。原位杂交分析显示，GCRG123在正常胃上皮和幽门腺中表达水平较高，但在肿瘤以及瘤旁区域的发育异常和大多数肠化生中表达水平较低。
在人胃黏膜中鉴定出一个核纤层样蛋白基因，它在胃癌及其癌前病变中表达下调。
