[烧伤血清对大鼠肠上皮细胞黏弹性及结构的影响]
[Effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells].
作者信息
Chen Jun, Tang Ao, Wu Ze-Zhi, Qin Jian, Xiao Guang-Xia
机构信息
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038 P.R. China.
出版信息
Zhonghua Shao Shang Za Zhi. 2003 Feb;19(1):32-4.
OBJECTIVE
To investigate the effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.
METHODS
The rat intestinal epithelial cell strain (IEC-6) was cultured and stimulated by burn serum. The changes of IECs before and after the stimulation were dynamically observed by cytoskeleton immunohistochemistry, ELISA and the measurement of cytomembranous viscoelasticity.
RESULTS
During the early stage of burn serum stimulation, the skeleton protein expression in IEC decreased obviously with weakened positive signals of microfilaments and microtubules and with decreased cellular elasticity.
CONCLUSION
The cytoskeleton injury could cause the increase of cellular fragility and the decrease of the viscoelasticity, which ultimately lead to the change of cellular biodynamics. These changes might directly participate the development of postburn intestinal epithelial injury.
目的
探讨烧伤血清对大鼠肠上皮细胞黏弹性及结构的影响。
方法
培养大鼠肠上皮细胞株（IEC-6），并用烧伤血清进行刺激。通过细胞骨架免疫组织化学、酶联免疫吸附测定法（ELISA）及细胞膜黏弹性测量，动态观察刺激前后肠上皮细胞的变化。
结果
在烧伤血清刺激早期，肠上皮细胞骨架蛋白表达明显降低，微丝和微管阳性信号减弱，细胞弹性下降。
结论
细胞骨架损伤可导致细胞脆性增加和黏弹性降低，最终引起细胞生物动力学改变。这些变化可能直接参与烧伤后肠上皮损伤的发生发展。
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