• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mutation and methylation of hMLH1 in gastric carcinomas with microsatellite instability.微卫星不稳定的胃癌中hMLH1的突变与甲基化
World J Gastroenterol. 2003 Apr;9(4):655-9. doi: 10.3748/wjg.v9.i4.655.
2
MSI-L gastric carcinomas share the hMLH1 methylation status of MSI-H carcinomas but not their clinicopathological profile.微卫星低度不稳定（MSI-L）胃癌与微卫星高度不稳定（MSI-H）癌具有相同的hMLH1甲基化状态，但临床病理特征不同。
Lab Invest. 2000 Dec;80(12):1915-23. doi: 10.1038/labinvest.3780201.
3
Concerted promoter hypermethylation of hMLH1, p16INK4A, and E-cadherin in gastric carcinomas with microsatellite instability.微卫星不稳定的胃癌中hMLH1、p16INK4A和E-钙黏蛋白启动子的协同高甲基化
J Pathol. 2003 May;200(1):23-31. doi: 10.1002/path.1325.
4
[Methylation of hMLH1 gene promoter in gastric carcinoma with microsatellite instability].[微卫星不稳定的胃癌中hMLH1基因启动子的甲基化]
Ai Zheng. 2005 Mar;24(3):273-7.
5
Hypermethylation of the hMLH1 gene promoter is associated with microsatellite instability in early human gastric neoplasia.hMLH1基因启动子的高甲基化与早期人类胃肿瘤中的微卫星不稳定性相关。
Oncogene. 2001 Jan 18;20(3):329-35. doi: 10.1038/sj.onc.1204104.
6
Methylation of the hMLH1 promoter but no hMLH1 mutations in sporadic gastric carcinomas with high-level microsatellite instability.散发性微卫星高度不稳定胃癌中hMLH1启动子甲基化但无hMLH1突变
Int J Cancer. 2000 Jul 15;87(2):200-3. doi: 10.1002/1097-0215(20000715)87:2<200::aid-ijc7>3.0.co;2-i.
7
hMLH1 promoter methylation and lack of hMLH1 expression in sporadic gastric carcinomas with high-frequency microsatellite instability.散发性高频微卫星不稳定胃癌中hMLH1启动子甲基化及hMLH1表达缺失
Cancer Res. 1999 Jan 1;59(1):159-64.
8
Hypermethylation of the hMLH1 gene promoter in solitary and multiple gastric cancers with microsatellite instability.微卫星不稳定的单发及多发胃癌中hMLH1基因启动子的高甲基化
Br J Cancer. 2002 Feb 12;86(4):564-7. doi: 10.1038/sj.bjc.6600076.
9
Evaluation of microsatellite instability, hMLH1 expression and hMLH1 promoter hypermethylation in defining the MSI phenotype of colorectal cancer.评估微卫星不稳定性、hMLH1表达及hMLH1启动子高甲基化在定义结直肠癌微卫星高度不稳定表型中的作用
Cancer Biol Ther. 2004 Jan;3(1):73-8. doi: 10.4161/cbt.3.1.590. Epub 2004 Jan 5.
10
Hypermethylation of the hMLH1 promoter in colon cancer with microsatellite instability.微卫星不稳定的结肠癌中hMLH1启动子的高甲基化
Cancer Res. 1998 Aug 1;58(15):3455-60.

引用本文的文献

1
TRIM37 contributes to malignant outcomes and CDDP resistance in gastric cancer.TRIM37促进胃癌的恶性结局和顺铂耐药。
J Cancer. 2021 Jan 1;12(2):316-325. doi: 10.7150/jca.47577. eCollection 2021.
2
Essential updates 2017/2018: Recent topics in the treatment and research of gastric cancer in Japan.2017/2018年度重要更新：日本胃癌治疗与研究的最新课题
Ann Gastroenterol Surg. 2019 Aug 27;3(6):581-591. doi: 10.1002/ags3.12284. eCollection 2019 Nov.
3
Microsatellite Instability and Altered Expressions of MLH1 and MSH2 in Gastric Cancer.胃癌中的微卫星不稳定性以及MLH1和MSH2的表达改变
Asian Pac J Cancer Prev. 2019 Feb 26;20(2):509-517. doi: 10.31557/APJCP.2019.20.2.509.
4
Overexpression of YEATS4 contributes to malignant outcomes in gastric carcinoma.YEATS4的过表达会导致胃癌出现恶性结果。
Am J Cancer Res. 2018 Dec 1;8(12):2436-2452. eCollection 2018.
5
Association Between hMLH1 Promoter Methylation and Risk of Gastric Cancer: A Meta-Analysis.hMLH1启动子甲基化与胃癌风险的关联：一项Meta分析
Front Physiol. 2018 Apr 17;9:368. doi: 10.3389/fphys.2018.00368. eCollection 2018.
6
Overexpression of PBK/TOPK relates to tumour malignant potential and poor outcome of gastric carcinoma.PBK/TOPK的过表达与胃癌的肿瘤恶性潜能及不良预后相关。
Br J Cancer. 2017 Jan 17;116(2):218-226. doi: 10.1038/bjc.2016.394. Epub 2016 Nov 29.
7
H. pylori infection is related to mitochondrial microsatellite instability in gastric carcinogenesis.幽门螺杆菌感染与胃癌发生中的线粒体微卫星不稳定性有关。
Infect Agent Cancer. 2016 Jul 12;11:30. doi: 10.1186/s13027-016-0078-5. eCollection 2016.
8
GFRA3 promoter methylation may be associated with decreased postoperative survival in gastric cancer.胶质细胞源性神经营养因子受体α3（GFRA3）启动子甲基化可能与胃癌术后生存率降低有关。
BMC Cancer. 2016 Mar 16;16:225. doi: 10.1186/s12885-016-2247-8.
9
Overexpression of denticleless E3 ubiquitin protein ligase homolog (DTL) is related to poor outcome in gastric carcinoma.无齿E3泛素蛋白连接酶同源物（DTL）的过表达与胃癌的不良预后相关。
Oncotarget. 2015 Nov 3;6(34):36615-24. doi: 10.18632/oncotarget.5620.
10
Overexpression of SMYD2 contributes to malignant outcome in gastric cancer.SMYD2的过表达促进胃癌的恶性结局。
Br J Cancer. 2015 Jan 20;112(2):357-64. doi: 10.1038/bjc.2014.543. Epub 2014 Oct 16.

本文引用的文献

1
DNA hypermethylation: when tumour suppressor genes go silent.DNA高甲基化：当肿瘤抑制基因沉默时。
Hum Genet. 2002 Aug;111(2):115-27. doi: 10.1007/s00439-002-0783-6. Epub 2002 Jul 16.
2
Treatment of cancer cells with methioninase produces DNA hypomethylation and increases DNA synthesis.用甲硫氨酸酶处理癌细胞会导致DNA低甲基化并增加DNA合成。
Cancer Res. 2002 Aug 15;62(16):4685-9.
3
CpG methylation modifies the genetic stability of cloned repeat sequences.CpG甲基化修饰克隆重复序列的遗传稳定性。
Genome Res. 2002 Aug;12(8):1246-56. doi: 10.1101/gr.74502.
4
Genetic and epigenetic modification of mismatch repair genes hMSH2 and hMLH1 in sporadic breast cancer with microsatellite instability.散发性微卫星不稳定型乳腺癌中错配修复基因hMSH2和hMLH1的遗传与表观遗传修饰
Oncogene. 2002 Aug 22;21(37):5696-703. doi: 10.1038/sj.onc.1205683.
5
Inverse relationship between APC gene mutation in gastric adenomas and development of adenocarcinoma.胃腺瘤中APC基因突变与腺癌发生之间的负相关关系。
Am J Pathol. 2002 Aug;161(2):611-8. doi: 10.1016/S0002-9440(10)64216-2.
6
Reactivating the expression of methylation silenced genes in human cancer.重新激活人类癌症中甲基化沉默基因的表达。
Oncogene. 2002 Aug 12;21(35):5496-503. doi: 10.1038/sj.onc.1205602.
7
5-Azacytidine and 5-aza-2'-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy.5-氮杂胞苷和5-氮杂-2'-脱氧胞苷作为DNA甲基化抑制剂：作用机制研究及其对癌症治疗的意义
Oncogene. 2002 Aug 12;21(35):5483-95. doi: 10.1038/sj.onc.1205699.
8
DNA methylation in cancer: too much, but also too little.癌症中的DNA甲基化：过多，但也过少。
Oncogene. 2002 Aug 12;21(35):5400-13. doi: 10.1038/sj.onc.1205651.
9
DNA methylation and cancer.DNA甲基化与癌症。
Oncogene. 2002 Aug 12;21(35):5358-60. doi: 10.1038/sj.onc.1205597.
10
Genetic instability in human mismatch repair deficient cancers.人类错配修复缺陷型癌症中的基因不稳定
Ann Genet. 2002 Apr-Jun;45(2):71-5. doi: 10.1016/s0003-3995(02)01115-2.

微卫星不稳定的胃癌中hMLH1的突变与甲基化

Mutation and methylation of hMLH1 in gastric carcinomas with microsatellite instability.

作者信息

Fang Dian-Chun, Wang Rong-Quan, Yang Shi-Ming, Yang Jian-Ming, Liu Hai-Feng, Peng Gui-Yong, Xiao Tian-Li, Luo Yuan-Hui

机构信息

Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

出版信息

World J Gastroenterol. 2003 Apr;9(4):655-9. doi: 10.3748/wjg.v9.i4.655.

DOI:10.3748/wjg.v9.i4.655
PMID:12679904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4611422/
Abstract

AIM

To appraise the correlation of mutation and methylation of hMSH1 with microsatellite instability (MSI) in gastric cancers.

METHODS

Mutation of hMLH1 was detected by Two-dimensional electrophoresis (Two-D) and DNA sequencing; Methylation of hMLH1 promoter was measured with methylation-specific PCR; MSI was analyzed by PCR-based methods.

RESULTS

Sixty-eight cases of sporadic gastric carcinoma were studied for mutation and methylation of hMLH1 promoter and MSI. Three mutations were found, two of them were caused by a single bp substitution and one was caused by a 2 bp substitution, which displayed similar Two-D band pattern. Methylation of hMLH1 promoter was detected in 11(16.2 %) gastric cancer. By using five MSI markers, MSI in at least one locus was detected in 17/68(25 %) of the tumors analyzed. Three hMLH1 mutations were all detected in MSI-H (>=2 loci, n=8), but no mutation was found in MSI-L (only one locus, n=9) or MSS (tumor lacking MSI or stable, n=51). Methylation frequency of hMLH1 in MSI-H (87.5 %, 7/8) was significantly higher than that in MSI-L (11.1 %, 1/9) or MSS (5.9 %, 3/51) (P<0.01-0.001), but no difference was found between MSI-L and MSS (P>0.05).

CONCLUSION

Both mutation and methylation of hMLH1 are involved in the MSI pathway but not related to the LOH pathway in gastric carcinogenesis.

摘要

目的

评估胃癌中hMSH1的突变和甲基化与微卫星不稳定性（MSI）的相关性。

方法

采用二维电泳（Two-D）和DNA测序检测hMLH1的突变；用甲基化特异性PCR检测hMLH1启动子的甲基化；通过基于PCR的方法分析MSI。

结果

对68例散发性胃癌进行hMLH1启动子的突变、甲基化及MSI研究。发现3个突变，其中2个由单个碱基替换引起，1个由2个碱基替换引起，它们显示出相似的二维电泳条带模式。11例（16.2%）胃癌检测到hMLH1启动子甲基化。使用5个MSI标记，在17/68（25%）分析的肿瘤中至少1个位点检测到MSI。3个hMLH1突变均在MSI-H（≥2个位点，n = 8）中检测到，但在MSI-L（仅1个位点，n = 9）或MSS（无MSI或稳定的肿瘤，n = 51）中未发现突变。hMLH1在MSI-H中的甲基化频率（87.5%，7/8）显著高于MSI-L（11.1%，1/9）或MSS（5.9%，3/51）（P<0.01 - 0.001），但MSI-L和MSS之间无差异（P>0.05）。

结论

hMLH1的突变和甲基化均参与胃癌发生的MSI途径，但与LOH途径无关。