α2b干扰素对慢性丙型病毒性肝炎患者外周血单个核细胞中mIL-2R诱导及HCV治疗的影响

Effect of alpha 2b interferon on inducement of mIL-2R and treatment of HCV in PBMC from patients with chronic viral hepatitis C.

作者信息

Wang Jian, Xiang Gui-Ju, Liu Bing-Xiang

机构信息

Department of Aetiology and Immunology, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China.

出版信息

World J Gastroenterol. 2003 Apr;9(4):751-4. doi: 10.3748/wjg.v9.i4.751.

DOI:10.3748/wjg.v9.i4.751

PMID:12679925

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4611443/

Abstract

AIM

To study the level of membrane interleukin-2 receptor (mIL-2R) on surface of peripheral blood mononuclear cells (PBMC) and the therapeutic efficacy of alpha 2b interferon on the treatment of HCV-RNA in PBMC of patients with chronic hepatitis C and to compare the negative rates of HCV-RNA in PBMC, HCV-RNA and anti-HCV in serum.

METHODS

Before and after treatment of alpha 2b interferon, the level of mIL-2R of patients with chronic hepatitis C was detected by biotin-streptavidin (BSA). The therapeutic group (26 cases) was treated with alpha 2b interferon (3 MU/d) and control therapeutic group (22 cases) was treated with routine drugs (VitC, aspartic acid). The total course of treatment with alpha 2b interferon and routine drug was six months and per course of the treatment was three months. The levels of HCV-RNA in PBMC, HCV-RNA and anti-HCV in serum were detected before and after a course of the treatment.

RESULTS

Before and after treatment of alpha 2b interferon and routine drugs, the levels of mIL-2R in silence stage were (3.44+/-0.77) % and (2.95+/-0.72) %, the levels of mIL-2R in inducement stage were (33.62+/-3.95) % and (30.04+/-3.73) %. There was a significant difference between two groups (P<0.01-P<0.05). After treatment of alpha 2b interferon with 3 MU/d for two courses of the treatment, the total negative rates of HCV-RNA in the PBMC and HCV-RNA, anti-HCV in serum were 42.31 % (11/26), 57.69 % (15/26), 65.38 %(17/26) respectively. After the treatment of routine drug, the negative rates of HCV-RNA in PBMC and HCV-RNA, anti-HCV in serum were 13.64 % (3/22), 22.73 % (5/22), 27.27 % (6/22) respectively. There was high significant difference in the group treated with alpha 2b interferon and the group treated with routine drugs (P<0.01-P<0.05).

CONCLUSION

The mIL-2R can be induced by alpha 2b interferon during the treatment. The alpha 2b interferon has a definite effect on the treatment of HCV-RNA in PBMC. The curative effect of alpha 2b interferon is better than that of the routine drugs.

摘要

目的

研究慢性丙型肝炎患者外周血单个核细胞（PBMC）表面膜白细胞介素-2受体（mIL-2R）水平及α2b干扰素对PBMC中丙型肝炎病毒核糖核酸（HCV-RNA）的治疗效果，并比较PBMC中HCV-RNA、血清中HCV-RNA及抗-HCV的阴转率。

方法

应用生物素-链霉亲和素（BSA）法检测慢性丙型肝炎患者在α2b干扰素治疗前后的mIL-2R水平。治疗组（26例）采用α2b干扰素（3MU/d）治疗，对照治疗组（22例）采用常规药物（维生素C、门冬氨酸）治疗。α2b干扰素及常规药物的总疗程均为6个月，每疗程3个月。在一个疗程治疗前后检测PBMC中HCV-RNA、血清中HCV-RNA及抗-HCV水平。

结果

α2b干扰素及常规药物治疗前后，静止期mIL-2R水平分别为（3.44±0.77）%和（2.95±0.72）%，诱导期mIL-2R水平分别为（33.62±3.95）%和（30.04±3.73）%。两组间差异有显著性（P<0.01-P<0.05）。应用3MU/d的α2b干扰素治疗两个疗程后，PBMC中HCV-RNA、血清中HCV-RNA及抗-HCV的总阴转率分别为42.31%（11/26）、57.69%（15/26）、65.38%（17/26）。常规药物治疗后，PBMC中HCV-RNA、血清中HCV-RNA及抗-HCV的阴转率分别为13.64%（3/22）、22.73%（5/22）、27.27%（6/22）。α2b干扰素治疗组与常规药物治疗组间差异有高度显著性（P<0.01-P<0.05）。