采用聚合酶链反应-限制性片段长度多态性技术对氟喹诺酮类药物敏感性降低的伤寒沙门菌和甲型副伤寒沙门菌的筛查方法
Screening method for Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones by PCR-restriction fragment length polymorphism.
作者信息
Hirose Kenji, Tamura Kazumichi, Watanabe Haruo
机构信息
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
出版信息
Microbiol Immunol. 2003;47(2):161-5. doi: 10.1111/j.1348-0421.2003.tb02800.x.
Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the mutations at codon Ser-83 and Asp-87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones.
对氟喹诺酮类药物敏感性降低（环丙沙星的 MIC 为 0.25 至 2 μg/ml）的伤寒沙门氏菌血清型 Typhi 和甲型副伤寒沙门氏菌血清型在 gyrA 基因的第 83 位密码子 Ser 或第 87 位密码子 Asp 处发生了突变。设计了一种通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的筛选方法，以筛选伤寒沙门氏菌血清型 Typhi 和甲型副伤寒沙门氏菌血清型临床分离株 gyrA 基因第 83 位密码子 Ser 和第 87 位密码子 Asp 处的突变。该方法成功筛选出了对氟喹诺酮类药物敏感性降低的伤寒沙门氏菌血清型 Typhi 和甲型副伤寒沙门氏菌血清型的 gyrA 突变。
Zotero 插件