WT1对牛磺酸转运体基因（TauT）的调控。

Regulation of taurine transporter gene (TauT) by WT1.

作者信息

Han Xiaobin, Chesney Russell W

机构信息

Department of Pediatrics, University of Tennessee Health Science Center, and the Children's Foundation Research Center at Le Bonheur Children's Medical Center, 50 North Dunlap, Memphis, TN 38103-4909, USA.

出版信息

FEBS Lett. 2003 Apr 10;540(1-3):71-6. doi: 10.1016/s0014-5793(03)00227-8.

DOI:10.1016/s0014-5793(03)00227-8

PMID:12681485

Abstract

In the present study we have demonstrated that WT1 (Wilms tumor suppressor gene) enhances the expression of TauT (taurine transporter gene) in human embryonic kidney 293 cells in a dose-dependent manner. TauT promoter activity was increased five-fold by cotransfection of a full-length TauT promoter-reporter construct with WT1. Electrophoretic mobility shift assays (EMSAs) using nuclear extracts from WT1-overexpressing 293 cells showed a putative WT1-binding site in the basal promoter region of TauT, which bound to WT1 in EMSAs. Mutation of this WT1 consensus sequence abolished binding of WT1. These results demonstrate that TauT may represent a downstream target gene of WT1 during renal development.

摘要

在本研究中，我们已经证明WT1（威尔姆斯肿瘤抑制基因）以剂量依赖的方式增强人胚肾293细胞中TauT（牛磺酸转运体基因）的表达。通过将全长TauT启动子 - 报告基因构建体与WT1共转染，TauT启动子活性增加了五倍。使用来自过表达WT1的293细胞的核提取物进行的电泳迁移率变动分析（EMSA）显示，在TauT的基础启动子区域存在一个推定的WT1结合位点，该位点在EMSA中与WT1结合。该WT1共有序列的突变消除了WT1的结合。这些结果表明，在肾脏发育过程中，TauT可能代表WT1的下游靶基因。