Rudolph Christoph, Schreier Peter H, Uhrig Joachim F
Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829 Cologne, Germany.
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4429-34. doi: 10.1073/pnas.0730832100. Epub 2003 Apr 7.
Plant viruses have a significant impact on agronomic losses worldwide. A new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. This peptide of 29 aa strongly interacts with the nucleocapsid proteins (N) of different tospoviruses. Transgenic Nicotiana benthamiana lines expressing the peptide fused to a carrier protein were challenged with five different tospoviruses that have a nucleocapsid protein interacting with the peptide. In the transgenic plants, strong resistance to tomato spotted wilt virus, tomato chlorotic spot virus, groundnut ring spot virus, and chrysanthemum stem necrosis virus was observed. This therefore demonstrates the feasibility of using peptide "aptamers" as an in vivo tool to control viral infection in higher plants.
植物病毒对全球农业产量损失有重大影响。本文提出了一种通过转基因表达显性干扰肽来培育抗病毒植物的新策略。这种由29个氨基酸组成的肽与不同番茄斑萎病毒属病毒的核衣壳蛋白(N)强烈相互作用。将表达与载体蛋白融合的该肽的转基因本氏烟草品系用五种不同的番茄斑萎病毒属病毒进行挑战,这些病毒的核衣壳蛋白与该肽相互作用。在转基因植物中,观察到对番茄斑萎病毒、番茄褪绿斑病毒、花生环斑病毒和菊花茎坏死病毒具有很强的抗性。因此,这证明了使用肽“适配体”作为体内工具来控制高等植物病毒感染的可行性。