Bengtsson Martin, Karlsson H Jonas, Westman Gunnar, Kubista Mikael
Department of Chemistry and Bioscience, Chalmers University of Technology 41296 Göteborg and TATAA Biocenter, Medicinaregatan 9E, 413 90 Göteborg, Sweden.
Nucleic Acids Res. 2003 Apr 15;31(8):e45. doi: 10.1093/nar/gng045.
The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BEBO concentrations between 0.1 and 0.4 micro M generated sufficient fluorescence signal without inhibiting the PCR. A comparison with the commonly used reporter dye SYBR Green I shows that the two dyes behave similarly in all important aspects.
对小沟结合型不对称花青染料4-[(3-甲基-6-(苯并噻唑-2-基)-2,3-二氢-(苯并-1,3-噻二唑)-2-亚甲基)]-1-甲基吡啶碘化物(BEBO)作为实时聚合酶链反应(PCR)中的序列非特异性标记物进行了测试。BEBO与双链DNA结合后荧光强度增加,使得能够在PCR循环的延伸阶段结束时测量荧光发射。0.1至0.4微摩尔之间的BEBO浓度产生了足够的荧光信号,且未抑制PCR。与常用的报告染料SYBR Green I的比较表明,这两种染料在所有重要方面表现相似。