人重组粒细胞集落刺激因子（HR-GCSF）对泌乳奶牛血常规的影响。

Effects of human recombinant granulocyte colony stimulating factor (HR-GCSF) on the hemogram of lactating dairy cattle.

作者信息

Cullor J. S., Smith W., Fairley N., Wood S. L., Dellinger J. D., Souza L.

机构信息

Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis, CA 95616.

出版信息

Vet Clin Pathol. 1990;19(1):9-12. doi: 10.1111/j.1939-165x.1990.tb00534.x.

DOI:10.1111/j.1939-165x.1990.tb00534.x

PMID:12684940

Abstract

Mastitis commonly occurs in the dairy cow and results in an influx of granulocytes into the mammary gland. Presently, colony stimulating factors have been isolated. One factor, granulocyte colony stimulating factor (GCSF), has been identified and reproduced using recombinant DNA technologies. In an experiment, lactating bovine were given a bacterially-synthesized human recombinant granulocyctic colony stimulating factor (Hr-GCSF) at a specified dose rate to monitor and characterize their hematological responses. Doses of HR-GCSF were administered subcutaneously and blood samples collected from the tail vein into vacutainer tubes. Results of the study indicated a toleration by the bovine for the HR-GCSF for the tested period, and that the HR-GCSF can stimulate a sustained elevation of circulating neutrophils.

摘要

乳腺炎常见于奶牛，会导致粒细胞流入乳腺。目前，已分离出集落刺激因子。其中一种因子，即粒细胞集落刺激因子（GCSF），已被鉴定并利用重组DNA技术进行了复制。在一项实验中，给泌乳牛以特定剂量率注射细菌合成的人重组粒细胞集落刺激因子（Hr-GCSF），以监测和表征它们的血液学反应。将Hr-GCSF皮下注射，并从尾静脉采集血样至真空采血管中。研究结果表明，在测试期间奶牛对Hr-GCSF具有耐受性，并且Hr-GCSF可刺激循环中性粒细胞持续升高。