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A型肉毒杆菌毒素对大鼠下尿路自主神经系统的影响。

Effect of botulinum toxin A on the autonomic nervous system of the rat lower urinary tract.

作者信息

Smith Christopher P, Franks Michael E, McNeil Brian K, Ghosh Rudrani, de Groat William C, Chancellor Michael B, Somogyi George T

机构信息

Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA.

出版信息

J Urol. 2003 May;169(5):1896-900. doi: 10.1097/01.ju.0000049202.56189.54.

Abstract

PURPOSE

The magnitude and duration of the effects of botulinum toxin A on acetylcholine (ACh) and norepinephrine release from the bladder and urethra of rats were measured using a radiochemical method.

MATERIALS AND METHODS

Saline (sham treatment) or botulinum toxin A was injected into the bladder (50 microl.) or urethra (30 microl.) in separate groups of animals. The release of 3H-norepinephrine or 14C-choline was measured at 2 time points after injection (5 or 30 days).

RESULTS

The fractional release of ACh in botulinum toxin A treated animals was significantly inhibited at higher frequencies of electrical field stimulation (20 Hz.) but not at lower frequencies (2 Hz.) 5 days after injection. However, ACh release recovered to sham injected values 30 days after toxin injection. No significant differences in the fractional release of norepinephrine from sham injected or botulinum toxin A bladders were observed. In contrast, norepinephrine release from the urethra was inhibited by botulinum toxin A for at least 30 days after injection. Similar to its effect on transmitter release in the bladder, botulinum toxin A inhibited norepinephrine release in the urethra at high (20 Hz.) but not at low (4 Hz.) electrical stimulation frequencies.

CONCLUSIONS

These data indicate that the clinical effects of botulinum toxin A on the lower urinary tract may vary depending on the site of injection and level of nerve activity.

摘要

目的

采用放射化学方法测定A型肉毒杆菌毒素对大鼠膀胱和尿道乙酰胆碱(ACh)及去甲肾上腺素释放的影响程度和持续时间。

材料与方法

将生理盐水(假处理)或A型肉毒杆菌毒素分别注射到不同组动物的膀胱(50微升)或尿道(30微升)中。在注射后2个时间点(5天或30天)测量3H-去甲肾上腺素或14C-胆碱的释放量。

结果

注射后5天,在较高电场刺激频率(20赫兹)下,A型肉毒杆菌毒素处理动物的ACh分数释放显著受到抑制,但在较低频率(2赫兹)下未受抑制。然而,毒素注射30天后,ACh释放恢复到假注射值。假注射组或A型肉毒杆菌毒素处理的膀胱中去甲肾上腺素的分数释放未观察到显著差异。相反,注射后至少30天,A型肉毒杆菌毒素抑制尿道去甲肾上腺素的释放。与其对膀胱递质释放的作用类似,A型肉毒杆菌毒素在高(20赫兹)但非低(4赫兹)电刺激频率下抑制尿道去甲肾上腺素的释放。

结论

这些数据表明,A型肉毒杆菌毒素对下尿路的临床作用可能因注射部位和神经活动水平而异。

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