黑色素瘤细胞从转铁蛋白同源物——黑素转铁蛋白的可溶形式中摄取铁。

Iron uptake by melanoma cells from the soluble form of the transferrin homologue, melanotransferrin.

作者信息

Food Michael R, Des Richardson R

机构信息

The Heart Research Institute, The Iron Metabolism and Chelation Group, Camperdown, Sydney, New South Wales, Australia.

出版信息

Redox Rep. 2002;7(5):279-82. doi: 10.1179/135100002125000794.

DOI:10.1179/135100002125000794

PMID:12688510

Abstract

Melanotransferrin (MTf) is a membrane-bound transferrin (Tf) homologue that can also exist in a soluble form (sMTf). Considering the high homology of MTf to Tf, it is possible to suggest that sMTf could bind to the high affinity transferrin receptor 1 (TfR1) or lower affinity TfR2. We have used sMTf labelled with 59Fe to examine its ability to donate Fe to cells. Our experiments demonstrate that sMTf is far less effective than Tf at donating Fe to cells and this does not occur via specific receptors. Indeed, the uptake of sMTf by cells occurred via a non-specific process (e.g. adsorptive pinocytosis).

摘要

黑色素转铁蛋白（MTf）是一种膜结合转铁蛋白（Tf）同源物，也可以以可溶性形式（sMTf）存在。鉴于MTf与Tf的高度同源性，有可能认为sMTf可以与高亲和力转铁蛋白受体1（TfR1）或低亲和力转铁蛋白受体2结合。我们使用用59Fe标记的sMTf来检测其向细胞供铁的能力。我们的实验表明，sMTf在向细胞供铁方面远不如Tf有效，且这不是通过特异性受体发生的。实际上，细胞对sMTf的摄取是通过非特异性过程（如吸附性胞饮作用）发生的。