一种新的互补DNA编码一种假定的类hRALY蛋白，即hRALYL。

A novel cDNA encodes a putative hRALY-like protein, hRALYL.

作者信息

Ji Chao-Neng, Chen Jin-Zhong, Xie Yi, Wang Shu, Qian Ji, Zhao Enpeng, Jin Wei, Wu Xiao-Zhou, Xu Wan-Xiang, Ying Kang, Mao Yu-Min

机构信息

State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.

出版信息

Mol Biol Rep. 2003 Mar;30(1):61-7. doi: 10.1023/a:1022295311177.

DOI:10.1023/a:1022295311177

PMID:12688537

Abstract

High throughput cDNA sequencing and 5'-rapid amplification of cDNA ends (5'RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.2. hRALYL might be a member of hnRNPC subfamily.

摘要

高通量cDNA测序和cDNA末端5'-快速扩增（5'RACE）分离出两个具有相同开放阅读框（ORF）的cDNA。用胎脑mRNA印迹进行的Northern印迹分析检测到两个转录本，长度分别为3.2 kb和2.2 kb。该ORF编码一个291个残基的推定蛋白，与hRALY和hnRNPC具有高度同源性。因此将其命名为类hRALY蛋白，即hRALYL。通过基于多组织cDNA（MTC）面板的RT-PCR检测表达模式。结果显示，hRALYL的转录本在人体组织中广泛表达，但强度不同。在脑中该转录本表达最高。Blast分析发现该cDNA对应于一个重叠群NT_008292，这表明该基因至少包含9个外显子，并将该基因定位在人类染色体8q21.13 - 8q21.2上。hRALYL可能是hnRNPC亚家族的成员。