Benes Francine M, Walsh John, Bhattacharyya Sujoy, Sheth Archna, Berretta Sabina
Laboratories for Structural Neuroscience, McLean Hospital, Belmont, MA 02478, USA.
Arch Gen Psychiatry. 2003 Apr;60(4):359-64. doi: 10.1001/archpsyc.60.4.359.
Apoptosis is thought to play a role in neuronal pathology in schizophrenia and bipolar disorder.
To test this hypothesis, the Klenow method for in situ end-labeling of single-stranded DNA breaks was applied to anterior cingulate cortex from 18 healthy controls, 18 schizophrenic subjects, and 10 bipolar subjects.
An unexpected reduction (71%) in Klenow-positive nuclei was found in schizophrenic but not in bipolar cortexes.
To our knowledge to date, this is the first demonstration that there is much less DNA fragmentation in individuals with schizophrenia than in healthy controls and bipolar subjects, which raises a key question as to whether this alteration represents an adaptive or nonadaptive change in the regulation of intracellular signaling and mitochondrial oxidative pathways associated with apoptosis.
细胞凋亡被认为在精神分裂症和双相情感障碍的神经元病理学中起作用。
为验证这一假设，采用Klenow法对18名健康对照者、18名精神分裂症患者和10名双相情感障碍患者的前扣带回皮质进行单链DNA断裂的原位末端标记。
在精神分裂症患者的皮质中发现Klenow阳性细胞核意外减少（71%），而在双相情感障碍患者的皮质中未发现。
据我们目前所知，这是首次证明精神分裂症患者的DNA片段化程度远低于健康对照者和双相情感障碍患者，这就提出了一个关键问题，即这种改变是否代表了与细胞凋亡相关的细胞内信号传导和线粒体氧化途径调节中的适应性或非适应性变化。
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