从感染HIV的供体的慢病毒载体转导的CD34+细胞中生成树突状细胞。

Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors.

作者信息

Gruber A, Chen I, Kuhen K L, Wheat J C, Law P, Wong-Staal F

机构信息

Department of Medicine, University of California, San Diego, La Jolla, California, USA.

出版信息

J Med Virol. 2003 Jun;70(2):183-6. doi: 10.1002/jmv.10376.

DOI:10.1002/jmv.10376

PMID:12696103

Abstract

Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34(+) cells from HIV-infected subjects can be transduced with a replication-incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34(+) cells from HIV-infected individuals were able to differentiate into dendritic cells with strong T-cell stimulatory capacity. Thus, cryopreserved CD34(+) cells from HIV-infected subjects may prove useful for immunotherapy for HIV/AIDS.

摘要

树突状细胞有望成为癌症和传染病免疫治疗的佐剂。我们证明，来自HIV感染受试者的大量冷冻保存的外周血CD34(+)细胞可以用表达HIV抗原的无复制能力的慢病毒载体进行转导。此外，来自HIV感染个体的未转导和转导的CD34(+)细胞能够分化为具有强大T细胞刺激能力的树突状细胞。因此，来自HIV感染受试者的冷冻保存的CD34(+)细胞可能被证明对HIV/AIDS的免疫治疗有用。