Klymchuk D O, Kordyum E L, Vorobyova T V, Brown C S, Chapman D K
Institute of Botany, Kyiv, Ukraine.
J Gravit Physiol. 2000 Jul;7(2):P79-80.
Phytoferritin is an iron-protein complex analogous to the ferritin found in mammalian, bacteria and fungi cells. Phytoferritin molecules are large proteins, about 10.5 nm in diameter, visualised in an electron microscope as discrete, electron dense particles with iron-containing core, where several thousand atoms of iron lie within the proteinaceous shell (apoferritin). In higher plants, a plastid stroma is the site of phytoferritin storage. Phytoferritin is seen in all types of plastids. It is considered to be a mechanism used by cells to store iron in a non-toxic form. Phytoferritin-bound iron may subsequently be used to form iron-containing components. It was shown that low levels of phytoferritin are synthesised in normal green leaves, whereas chlorotic leaves do not have a measurable amount of phytoferritin and leaves of iron-loaded seedlings contain a high level of total iron, and phytoferritin well-filled by iron. Phytoferritin accumulation was observed in photosynthetic inactivity chloroplasts during senescence and disease. In this study we analised the effects of microgravity and ethylene on production of phytoferritin in the root cap columella cells of soybean seedlings.
植物铁蛋白是一种铁 - 蛋白质复合物，类似于在哺乳动物、细菌和真菌细胞中发现的铁蛋白。植物铁蛋白分子是大蛋白质，直径约为10.5纳米，在电子显微镜下呈现为离散的、电子致密的颗粒，具有含铁核心，其中数千个铁原子位于蛋白质外壳（脱铁铁蛋白）内。在高等植物中，质体基质是植物铁蛋白的储存部位。在所有类型的质体中都能看到植物铁蛋白。它被认为是细胞以无毒形式储存铁的一种机制。与植物铁蛋白结合的铁随后可用于形成含铁成分。研究表明，正常绿叶中合成的植物铁蛋白水平较低，而黄化叶中没有可测量的植物铁蛋白，铁负载幼苗的叶片含有高水平的总铁，且植物铁蛋白中铁含量充足。在衰老和疾病过程中，在光合无活性的叶绿体中观察到了植物铁蛋白的积累。在本研究中，我们分析了微重力和乙烯对大豆幼苗根冠柱细胞中植物铁蛋白产生的影响。
