Pellestor Franck, Andréo Brigitte, Taneja Krihan, Williams Brett
CNRS-UPR 1142, IGH, 141 rue de la Cardonille, F-34396 Montpellier cedex 5, France.
Eur J Hum Genet. 2003 Apr;11(4):337-41. doi: 10.1038/sj.ejhg.5200958.
Peptide nucleic acids (PNAs) are a relatively new class of synthetic DNA mimics based on a peptide-like backbone. Since their introduction, PNA probes have become established as an efficient variation on the standard FISH procedure for chromosomal identification. In this report we have experimented with centromeric PNA probes on human sperm preparations. Both NaOH and DTT sperm decondensation procedures have been tested and comparative estimates of disomies X, Y and 1 have been performed in sperm from two donors using PNA, FISH and PRINS techniques. Similar results were obtained with the three methods, demonstrating the efficiency of PNA probes in the analysis of human sperm. The fast kinetics, stability and high specificity of PNA probes make PNA-based methodologies very valuable for in situ cytogenetic investigations.
肽核酸（PNA）是一类相对较新的基于肽样主链的合成DNA模拟物。自引入以来，PNA探针已成为用于染色体鉴定的标准荧光原位杂交（FISH）程序的一种有效变体。在本报告中，我们对人类精子样本进行了着丝粒PNA探针实验。我们测试了氢氧化钠（NaOH）和二硫苏糖醇（DTT）精子解聚程序，并使用PNA、FISH和引物原位标记（PRINS）技术对两名供体的精子中的X、Y和1号染色体三体进行了比较评估。三种方法获得了相似的结果，证明了PNA探针在人类精子分析中的有效性。PNA探针的快速动力学、稳定性和高特异性使得基于PNA的方法在原位细胞遗传学研究中非常有价值。
