Valério Elisabete, Gadanho Mário, Sampaio José Paulo
Centro de Recursos Microbiológicos, Secção Autónoma de Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
FEMS Yeast Res. 2002 Mar;2(1):9-16. doi: 10.1111/j.1567-1364.2002.tb00063.x.
This report presents the description of a new Sporobolomyces species, Sp. odoratus sp. nov. The new species was characterized by DNA fingerprinting using the micro/minisatellite-primed PCR approach (MSP-PCR). A phylogenetic analysis using the D1/D2 region of the 26S rDNA revealed that Sp. odoratus belongs to a clade that includes Rhodosporidium fluviale and Sporidiobolus ruineniae. An integrated comparison with related species is presented. A number of presumptive strains of S. ruineniae were also investigated using the MSP-PCR method and 26S rDNA sequence analysis. Mating experiments revealed, for the first time, that S. ruineniae includes heterothallic strains, besides those already known to be self-fertile.
本报告描述了一种新的掷孢酵母属物种,即香掷孢酵母(Sporobolomyces odoratus sp. nov.)。该新物种通过使用微卫星/小卫星引物PCR方法(MSP-PCR)进行DNA指纹分析来表征。利用26S rDNA的D1/D2区域进行的系统发育分析表明,香掷孢酵母属于一个进化枝,其中包括河流红冬孢酵母(Rhodosporidium fluviale)和鲁氏掷孢酵母(Sporidiobolus ruineniae)。本文还对相关物种进行了综合比较。此外,还使用MSP-PCR方法和26S rDNA序列分析对一些推定的鲁氏掷孢酵母菌株进行了研究。交配实验首次揭示,除了已知的自育菌株外,鲁氏掷孢酵母还包括异宗配合菌株。