Schneck P K, Staudenbauer W L, Hofschneider P H
Nucleic Acids Res. 1976 Mar;3(3):599-613. doi: 10.1093/nar/3.3.599.
Cell-free extracts from Escherichia coli contain a DNA polymerase activity resistant to SH-blocking agents, which is capable of synthesizing complementary strand DNA on a circular M-13 DNA template by extension of RNA primers. This activity is considered to be identical with DNA polymerase I (or some altered form of this enzyme) since it is missing in extracts from po1A- cells. DNA synthesis in the presence of SH-blocking agents occurs at a reduced rate as compared to untreated controls and leads to the formation of DNA chains of defined size (0.4-0.5 genome's length). It is concluded that efficient M-13 duplex DNA synthesis requires the cooperation of both DNA polymerase I and III.
大肠杆菌的无细胞提取物含有一种对SH阻断剂有抗性的DNA聚合酶活性,该活性能够通过RNA引物的延伸在环状M-13 DNA模板上合成互补链DNA。这种活性被认为与DNA聚合酶I(或该酶的某种改变形式)相同,因为在po1A-细胞的提取物中不存在。与未处理的对照相比,在SH阻断剂存在下的DNA合成速率降低,并导致形成确定大小(0.4-0.5个基因组长度)的DNA链。结论是高效的M-13双链DNA合成需要DNA聚合酶I和III的共同作用。