Bittencourt Nelson S, Gibbs Peter E, Semir João
Departamento de Botânica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil.
Ann Bot. 2003 Jun;91(7):827-34. doi: 10.1093/aob/mcg088. Epub 2003 Mar 28.
The reproductive biology of Spathodea campanulata was investigated by means of hand-pollination experiments, observations of pollen tube growth using fluorescence microscopy, and serial sections of ovules in selfed and crossed pistils. Only cross-pollinated flowers developed fruits, and all selfed flowers abscised within 3-4 d. However, self pollen tubes grew successfully to the ovary, penetrating and fertilizing the majority of ovules by 48 h, indicating that S. campanulata is a species with late-acting self-incompatibility. The incidences of ovule penetration, fertilization and endosperm initiation were all significantly slower in selfed vs. crossed pistils, although no other signs of malfunctioning were detected. The possible role of such slow self pollen tube effectiveness as a recognition event is discussed within the context of the slow but not entirely suppressed self pollen tube growth reported for some species with conventional homomorphic self-incompatibility.
通过人工授粉实验、利用荧光显微镜观察花粉管生长以及对自交和杂交雌蕊中的胚珠进行连续切片，对火焰树的生殖生物学进行了研究。只有异花授粉的花朵能发育成果实，所有自花授粉的花朵在3 - 4天内脱落。然而，自花花粉管成功生长至子房，到48小时时穿透并使大多数胚珠受精，这表明火焰树是一种具有晚期自交不亲和性的物种。与杂交雌蕊相比，自交雌蕊中胚珠穿透、受精和胚乳起始的发生率均显著较慢，尽管未检测到其他功能异常的迹象。在一些具有传统同型自交不亲和性的物种中，报道了自花花粉管生长缓慢但并非完全受抑制的情况，在此背景下讨论了这种缓慢的自花花粉管有效性作为识别事件的可能作用。
