DNA胀泡BhC4-1启动子中调控区域的鉴定

Identification of regulatory regions in the DNA puff BhC4-1 promoter.

作者信息

Monesi N, Basso L R, Paçó-Larson M L

机构信息

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.

出版信息

Insect Mol Biol. 2003 Jun;12(3):247-54. doi: 10.1046/j.1365-2583.2003.00408.x.

DOI:10.1046/j.1365-2583.2003.00408.x

PMID:12752658

Abstract

The mechanisms that control DNA puff BhC4-1 expression in the salivary gland of sciarid late larvae have been shown to be conserved in Drosophila. By analysing Drosophila transformed with constructs carrying progressive deletions of the BhC4-1 promoter fragment (-3314/+40) fused to the lacZ reporter gene we show that the elements required for the correct BhC4-1-lacZ developmental regulation in prepupal salivary glands are contained in a 226 bp fragment (-186/+40). Also, interestingly, this study identified a 67 bp fragment (-253/-187) that activates BhC4-1-lacZ expression specifically in the ring gland.

摘要

已证明，在蕈蚊晚期幼虫唾液腺中控制DNA胀泡BhC4-1表达的机制在果蝇中是保守的。通过分析用携带与lacZ报告基因融合的BhC4-1启动子片段（-3314/+40）进行渐进缺失构建体转化的果蝇，我们发现，蛹前唾液腺中BhC4-1-lacZ正确发育调控所需的元件包含在一个226 bp片段（-186/+40）中。同样有趣的是，这项研究鉴定出一个67 bp片段（-253/-187），它能特异性激活环腺中BhC4-1-lacZ的表达。