γ-促黑素在体外可增加细胞内cAMP积累和促性腺激素释放激素释放，在体内可增加促黄体生成素释放。

gamma-MSH increases intracellular cAMP accumulation and GnRH release in vitro and LH release in vivo.

作者信息

Stanley S A, Davies S, Small C J, Gardiner J V, Ghatei M A, Smith D M, Bloom S R

机构信息

Endocrine Unit, Division of Investigative Science, ICSM, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK.

出版信息

FEBS Lett. 2003 May 22;543(1-3):66-70. doi: 10.1016/s0014-5793(03)00402-2.

DOI:10.1016/s0014-5793(03)00402-2

PMID:12753907

Abstract

The roles of the melanocortin 3 receptor (MC3-R) and its agonist, gamma(2)-melanocyte-stimulating hormone (gamma(2)-MSH) in the regulation of the hypothalamo-pituitary-gonadal (HPG) axis are poorly understood. Here we show gamma(2)-MSH stimulated intracellular cAMP accumulation and gonadotrophin-releasing hormone (GnRH) secretion in the immortalised GnRH cell line GT(1)-7. The MC3/4-R antagonist Agrp blocked these actions. Reverse transcriptase polymerase chain reaction demonstrated GT(1)-7 cells express MC3-R mRNA. gamma(2)-MSH also stimulated GnRH release from hypothalamic explants. In vivo, gamma(2)-MSH administration into the medial preoptic area significantly increased plasma luteinising hormone. MC3-R and gamma(2)-MSH may modulate the HPG axis.

摘要

黑皮质素3受体（MC3-R）及其激动剂γ(2)-促黑素细胞激素（γ(2)-MSH）在调节下丘脑-垂体-性腺（HPG）轴中的作用目前尚不清楚。在此我们发现，γ(2)-MSH可刺激永生化促性腺激素释放激素（GnRH）细胞系GT(1)-7内的细胞内环磷酸腺苷（cAMP）积累及GnRH分泌。MC3/4-R拮抗剂阿黑皮素原（Agrp）可阻断这些作用。逆转录聚合酶链反应表明GT(1)-7细胞表达MC3-R信使核糖核酸（mRNA）。γ(2)-MSH还可刺激下丘脑外植体释放GnRH。在体内，向视前内侧区注射γ(2)-MSH可显著提高血浆黄体生成素水平。MC3-R和γ(2)-MSH可能对HPG轴具有调节作用。