Gustafsson Margaretha K S, Terenina Nadezhda B, Reuter Maria, Movsessian Sergei O
Department of Biology, Abo Akademi University, Artillerigatan 6, 20520, Abo, Finland.
Parasitol Res. 2003 Jun;90(2):148-52. doi: 10.1007/s00436-003-0826-5. Epub 2003 Feb 19.
We studied the pattern of cGMP immunostaining (IS) after stimulation with a nitric oxide donor in the presence of an inhibitor of phosphodiesterase in adult Hymenolepis diminuta. cGMP-IS was detected in the peripheral nervous system, especially in nerve fibres close to the body muscle fibres. cGMP-IS also occurred in terminals beneath the basal lamina of the tegument and between the muscle fibres of the suckers. The pattern of cGMP-IS was compared to that of 5-HT-IS and GYIRFamide-IS. TRITC-conjugated phalloidin was used to stain the musculature.
我们研究了在成年微小膜壳绦虫中,在磷酸二酯酶抑制剂存在的情况下,用一氧化氮供体刺激后cGMP免疫染色(IS)的模式。在周围神经系统中检测到cGMP-IS,特别是在靠近身体肌肉纤维的神经纤维中。cGMP-IS也出现在皮层基膜下方的终末以及吸盘肌肉纤维之间。将cGMP-IS的模式与5-HT-IS和GYIRFamide-IS的模式进行了比较。使用TRITC偶联的鬼笔环肽对肌肉组织进行染色。