Wang Dong-mei, Zhu Qi-ying, Ding Li, Ma Dong
Department of Obstetrics and Gynecology, First Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830054, China.
Zhonghua Fu Chan Ke Za Zhi. 2003 Jan;38(1):5-7.
To investigate the changes of gene p53, bax and bcl-2 expression and to study the role on cell apoptosis in placenta through apoptosis in placenta with intrahepatic cholestasis of pregnancy (ICP).
TdT-mediated dUTP nick end labeling (TUNEL) and immuno-histochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index and the expression of p53, bax and bcl-2 in placenta tissues.
The apoptosis index in placental cytotrophoblast, syncytiotrophoblast, decidual cells and mediate cell on ICP group was significantly higher than that in control group (P < 0.01). The expression of p53 was higher than those of control. Bax expression in syncytoitrophoblast, decidual cell and mediate cell in ICP group was higher (P < 0.01), but the expression of bax in placental cytotrophoblast cell was not significantly higher (P > 0.05). The expression of bcl-2 of these positions in ICP group was lower than that in control group (P < 0.01) p53, bax and bcl-2 expressions in (chi +/- s)% in the syncytoitrophoblast cells of placenta with ICP is 75.9 +/- 8.2, 65.8 +/- 13.8, 45.3 +/- 11.3.
The placental dysfunction may closely relate to the apoptosis. Abnormal overexpression of p53, bax and the low expression of bcl-2 on placental tissue of ICP was the main reason of placental apoptosis.
通过检测妊娠肝内胆汁淤积症（ICP）患者胎盘组织细胞凋亡情况，探讨p53、bax和bcl-2基因表达变化及其在胎盘细胞凋亡中的作用。
采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）和免疫组织化学方法，对31例ICP患者胎盘组织和31例正常胎盘组织进行凋亡指数及p53、bax和bcl-2表达检测。
ICP组胎盘细胞滋养层细胞、合体滋养层细胞、蜕膜细胞及间质细胞凋亡指数明显高于对照组（P<0.01）；p53表达高于对照组；ICP组合体滋养层细胞、蜕膜细胞及间质细胞bax表达升高（P<0.01），而细胞滋养层细胞bax表达差异无统计学意义（P>0.05）；ICP组上述部位bcl-2表达低于对照组（P<0.01）。ICP患者胎盘合体滋养层细胞中p53、bax和bcl-2表达（均数±标准差）分别为75.9±8.2、65.8±13.8、45.3±11.3。
胎盘功能障碍可能与细胞凋亡密切相关，ICP患者胎盘组织p53、bax异常高表达及bcl-2低表达是胎盘细胞凋亡的主要原因。
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