肠出血性大肠杆菌O157:H-和O157:H7中的细胞致死膨胀毒素基因簇：特征与进化考量
Cytolethal distending toxin gene cluster in enterohemorrhagic Escherichia coli O157:H- and O157:H7: characterization and evolutionary considerations.
作者信息
Janka Andreas, Bielaszewska Martina, Dobrindt Ulrich, Greune Lilo, Schmidt M Alexander, Karch Helge
机构信息
Institut für Hygiene, Universitätsklinikum Münster, Germany.
出版信息
Infect Immun. 2003 Jun;71(6):3634-8. doi: 10.1128/IAI.71.6.3634-3638.2003.
Abstract
We identified a cytolethal distending toxin (cdt) gene cluster in 87, 6, and 0% of sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H(-), EHEC O157:H7, and E. coli O55:H7/H(-) strains, respectively. The toxin was expressed by the wild-type EHEC O157 strains and by a cdt-containing cosmid from a library of SF EHEC O157:H(-) strain 493/89. The cdt flanks in strain 493/89 were homologous to bacteriophages P2 and lambda. Our data demonstrate that cdt, encoding a potential virulence factor, is present in the EHEC O157 complex and suggest that cdt may have been acquired by phage transduction.
摘要
我们分别在87%的山梨醇发酵型（SF）肠出血性大肠杆菌（EHEC）O157:H(-)菌株、6%的EHEC O157:H7菌株和0%的大肠杆菌O55:H7/H(-)菌株中鉴定出一个细胞致死膨胀毒素（cdt）基因簇。该毒素可由野生型EHEC O157菌株以及来自SF EHEC O157:H(-)菌株493/89文库的含cdt黏粒表达。菌株493/89中的cdt侧翼与噬菌体P2和λ同源。我们的数据表明，编码一种潜在毒力因子的cdt存在于EHEC O157复合体中，并提示cdt可能是通过噬菌体转导获得的。
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