Feng Y, Zhang B, Tan H, Su H, He W
Department of Otorhinolaryngology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Aug;36(4):264-6.
To study the role and the clinical significance of dendritic cell infiltration against tumor cells in patients with laryngeal squamous cell carcinoma.
Immunohistochemical method using S-100 protein antibody was employed to detect dendritic cells (DC) on paraffin-embedded tissue sections from 23 patients with laryngeal squamous cell carcinomas. The relationships among the density of dendritic cell infiltration in laryngeal carcinomas and cervical lymphatic metastasis, pericancerous lymphocytic reaction and other clinicopathologic parameters were observed.
The numbers of dendritic cells infiltrating among laryngeal carcinoma cells in patients with no cervical lymph metastasis was significantly larger than those with cervical lymphatic metastasis (t = 4.889, P < 0.01). Significant increase DC infiltration among laryngeal carcinoma cells was found in the group with intensively positive reaction of pericancerous lymphocyte than in the medium and weakly positive groups. The number of infiltrating dendritic cells in patients who had survived more than 5 years was significantly larger than those survived less than 5 years (t = 4.423, P < 0.01). The numbers of the infiltrating dendritic cells in patients with well-differentiated squamous cell carcinoma and poorly differentiated squamous cells carcinoma were 2.2 +/- 1.07 and 14.6 +/- 7.14 respectively, which were significantly different (q = 4.532, P < 0.05).
The study showed that DC infiltration among laryngeal squamous carcinoma cells played an important role in the host immune reaction against tumor. It indicated that when the density of infiltrating DC is higher, the patients had less chance of cervical lymph metastasis and may have a longer survival time. There was a coordinative effect between DC infiltration in tumor cells and pericancerous lymphocyte reaction. Thus, the DC infiltration among laryngeal squamous cells could be used as an index of prognosis.
探讨树突状细胞浸润在喉鳞状细胞癌患者中对肿瘤细胞的作用及临床意义。
采用免疫组织化学方法，用S-100蛋白抗体检测23例喉鳞状细胞癌石蜡包埋组织切片中的树突状细胞（DC）。观察喉癌中树突状细胞浸润密度与颈部淋巴结转移、癌周淋巴细胞反应及其他临床病理参数之间的关系。
无颈部淋巴结转移患者的喉癌细胞间浸润的树突状细胞数量明显多于有颈部淋巴结转移者（t = 4.889，P < 0.01）。癌周淋巴细胞反应强阳性组的喉癌细胞间DC浸润明显多于中度阳性和弱阳性组。生存超过5年患者的浸润树突状细胞数量明显多于生存不足5年者（t = 4.423，P < 0.01）。高分化鳞状细胞癌和低分化鳞状细胞癌患者的浸润树突状细胞数量分别为2.2±1.07和14.6±7.14，差异有统计学意义（q = 4.532，P < 0.05）。
研究表明，喉鳞状癌细胞间的DC浸润在宿主抗肿瘤免疫反应中起重要作用。提示浸润DC密度越高，患者颈部淋巴结转移机会越少，生存时间可能越长。肿瘤细胞中的DC浸润与癌周淋巴细胞反应之间存在协同效应。因此，喉鳞状细胞间的DC浸润可作为预后指标。
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