[上呼吸道肥大细胞中雌二醇受体的表达]
[Expression of estradiol receptors in mast cells of the upper airway].
作者信息
Zhao X, Dong Z, Yang Z
机构信息
Department of ENT & Head-Neck Surgery, Third Teaching Hospital, Bethune University of Medical Sciences, Changchun 130031.
出版信息
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1999 Jun;34(3):135-7.
OBJECTIVE
To examine the expression of estrogen receptors in mast cells, CD4+, CD8+ and CD68+ cells in human nasal polyps.
METHODS
Immunohistochemical and dual immunostaining methods were used with mouse monoclonal antibodies against human estrogen receptors, a range of human immune cell types included CD4+ (helper T-cells), CD8+ (suppressor/cytotoxic T-cells), CD68+ (Macrophages) cells and mast cells.
RESULTS
Coincident with the known features of human nasal polyps, all the immune/inflammatory cell types examined were clearly detected with corresponding monoclonal antibodies. The cells positive for estrogen receptor were morphologically similar to the mast cells but distinct from CD4+, CD8+ or CD68+ cells. The cells stained for estrogen receptors by dual immunostaining coincided exactly with cells labeled as mast cells but differed from CD4+, CD8+ or CD68+ cells.
CONCLUSION
Estrogen receptors express only in mast cells, but not in CD4+, CD8+ or CD68+ cells in the human upper airway.
目的
检测人鼻息肉中肥大细胞、CD4⁺、CD8⁺和CD68⁺细胞中雌激素受体的表达情况。
方法
采用免疫组织化学和双重免疫染色方法，使用抗人雌激素受体的小鼠单克隆抗体，检测一系列人类免疫细胞类型，包括CD4⁺（辅助性T细胞）、CD8⁺（抑制性/细胞毒性T细胞）、CD68⁺（巨噬细胞）细胞和肥大细胞。
结果
与已知的人鼻息肉特征相符，所有检测的免疫/炎症细胞类型均能用相应的单克隆抗体清晰检测到。雌激素受体阳性细胞在形态上与肥大细胞相似，但与CD4⁺、CD8⁺或CD68⁺细胞不同。双重免疫染色显示，雌激素受体染色的细胞与标记为肥大细胞的细胞完全一致，但与CD4⁺、CD8⁺或CD68⁺细胞不同。
结论
雌激素受体仅在人上呼吸道的肥大细胞中表达，而不在CD4⁺、CD8⁺或CD68⁺细胞中表达。
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