Panina Ekaterina M, Vitreschak Alexey G, Mironov Andrey A, Gelfand Mikhail S
Graduate Program in Molecular, Cellular and Integrative Life Sciences, 172 Molecular Science Building, University of California at Los Angeles, Los Angeles, CA 90095-1570, USA.
FEMS Microbiol Lett. 2003 May 28;222(2):211-20. doi: 10.1016/S0378-1097(03)00303-3.
Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococcus species. New T-boxes were found upstream of aromatic amino acid biosynthesis and transport genes in the Bacillus/Clostridium group. The substrate specificity of proteins from the PabA/TrpG family was assigned based on metabolic reconstruction and analysis of regulatory signals and phylogenetic patterns. New candidate tryptophan transporters were identified; their specificity was predicted by analysis of T-box regulatory sites. Comparison of all available genomes shows that regulation of genes of the aromatic amino acid biosynthesis pathway is quite labile and involves at least four regulatory systems, two at the DNA level and two more involving competition of alternative RNA secondary structures for transcription and/or translation regulation at the RNA level.
计算比较技术被应用于革兰氏阳性菌中芳香族氨基酸调节子的分析。在链球菌属和乳球菌属中鉴定出了3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶和莽草酸激酶基因的一个新的候选转录调控信号。在芽孢杆菌/梭菌组的芳香族氨基酸生物合成和转运基因上游发现了新的T-盒。基于代谢重建以及调控信号和系统发育模式的分析，确定了来自PabA/TrpG家族蛋白质的底物特异性。鉴定出了新的候选色氨酸转运蛋白；通过分析T-盒调控位点预测了它们的特异性。对所有可用基因组的比较表明，芳香族氨基酸生物合成途径基因的调控相当不稳定，涉及至少四种调控系统，两种在DNA水平，另外两种涉及在RNA水平上替代RNA二级结构对转录和/或翻译调控的竞争。
