异常布勒酵母新种和拟多变布勒酵母新种，来自中国云南的两个形成掷孢子的新酵母物种。

Bullera anomala sp. nov. and Bullera pseudovariabilis sp. nov., two new ballistoconidium-forming yeast species from Yunnan, China.

作者信息

Bai Feng-Yan, Takashima Masako, Zhao Jian-Hua, Jia Jian-Hua, Nakase Takashi

机构信息

Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Antonie Van Leeuwenhoek. 2003;83(3):257-63. doi: 10.1023/a:1023382311865.

DOI:10.1023/a:1023382311865

PMID:12776921

Abstract

Two ballistoconidium-forming yeast strains, CH 2.504 and CH 2.509, were isolated from the wilting leaves of Parthenocissus sp. collected in Yunnan, China in 1996. These two strains were assigned to the genus Bullera Derx by the characteristics of forming rotationally symmetric ballistoconidia, containing xylose in the cell hydrolysates and having Q-10 as the major ubiquinone. Molecular phylogenetic analysis based on small subunit ribosomal RNA gene (18S rDNA) and internal transcribed spacer (ITS) region sequences revealed that CH 2.504 is closely related to B. variabilis Nakase & Suzuki, while CH 2.509 is located in a clearly separate clade among the described Bullera species and related taxa. The results indicated that CH 2.509 represents a new species, for which Bullera anomala sp. nov. is the proposed name (type strain AS 2.2094T). Further DNA-DNA reassociation data showed that CH 2.504 also represents a distinct species, for which the name Bullera pseudovariabilis sp. nov. is given (type strain AS 2.2092T).

摘要

1996年，从中国云南采集的爬山虎枯萎叶片中分离出两株形成掷孢子的酵母菌株CH 2.504和CH 2.509。根据形成旋转对称掷孢子、细胞水解产物中含有木糖以及以Q-10作为主要泛醌的特征，这两株菌株被归为布勒酵母属（Bullera Derx）。基于小亚基核糖体RNA基因（18S rDNA）和内部转录间隔区（ITS）序列的分子系统发育分析表明，CH 2.504与多变布勒酵母（B. variabilis Nakase & Suzuki）密切相关，而CH 2.509位于已描述的布勒酵母属物种和相关类群中一个明显独立的分支中。结果表明，CH 2.5代表一个新物种，提议将其命名为异常布勒酵母（Bullera anomala sp. nov.）（模式菌株AS 2.2094T）。进一步的DNA-DNA杂交数据表明，CH 2.504也代表一个独特的物种，将其命名为拟多变布勒酵母（Bullera pseudovariabilis sp. nov.）（模式菌株AS 2.2092T）。