Narita Motoshi, Murata Taku, Shimizu Kasumi, Sugiyama Takeo, Nakagawa Toshiyuki, Manganiello Vincent C, Tagawa Toshiro
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Tsu, Japan.
Anticancer Drugs. 2003 Jun;14(5):377-81. doi: 10.1097/00001813-200306000-00009.
Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that HOSM-1 cells, an osteosarcoma cell line established from human mandible, expressed mRNA for osteoblastic markers, such as alkaline phosphatase, osteonectin, osteocalcin and parathyroid hormone receptor, thus exhibiting an osteoblastic phenotype. We have investigated a possible role of cyclic nucleotide phosphodiesterases (PDEs) in osteosarcoma cells. RT-PCR analysis revealed that HOSM-1 cells expressed mRNA for PDE4A, 4B and 4C. In addition, rolipram, a specific inhibitor of PDE4, inhibited HOSM-1 cell proliferation. The finding that PDE4 is involved in proliferation of osteosarcoma cells suggests the possibility that PDE4 may be a new target for antitumor therapy.
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