HCN通道在视网膜双极细胞中呈差异性表达，并集中于突触终末。

HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals.

作者信息

Müller Frank, Scholten Alexander, Ivanova Elena, Haverkamp Silke, Kremmer Elisabeth, Kaupp U Benjamin

机构信息

Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, D-52425 Jülich, Germany.

出版信息

Eur J Neurosci. 2003 May;17(10):2084-96. doi: 10.1046/j.1460-9568.2003.02634.x.

DOI:10.1046/j.1460-9568.2003.02634.x

PMID:12786975

Abstract

Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels codetermine the integrative behaviour of neurons and shape their response to synaptic stimulation. We used immunohistochemistry and patch-clamp recording to study the composition and distribution of HCN channels in the rat retina. All four HCN channel isoforms (HCN1-4) are expressed differentially in the retina. In particular, different classes of bipolar cells have a different inventory of HCN channels. We found no evidence for the formation of heterooligomeric HCN channels. HCN channels are densely clustered at synaptic terminals of bipolar cells and photoreceptors. This suggests that HCN channels are involved in the control of transmitter release.

摘要

超极化激活的环核苷酸门控（HCN）通道共同决定神经元的整合行为，并塑造其对突触刺激的反应。我们使用免疫组织化学和膜片钳记录来研究大鼠视网膜中HCN通道的组成和分布。所有四种HCN通道亚型（HCN1 - 4）在视网膜中的表达存在差异。特别是，不同类型的双极细胞具有不同的HCN通道组合。我们没有发现形成异源寡聚体HCN通道的证据。HCN通道密集地聚集在双极细胞和光感受器的突触末端。这表明HCN通道参与了神经递质释放的控制。