日本牙鲆（Paralichthys olivaceus）IgD编码cDNA的克隆与鉴定

Cloning and characterisation of a cDNA encoding Japanese flounder Paralichthys olivaceus IgD.

作者信息

Hirono Ikuo, Nam Bo-Hye, Enomoto Jyungo, Uchino Kanako, Aoki Takashi

机构信息

Laboratory of Genetics, Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.

出版信息

Fish Shellfish Immunol. 2003 Jul;15(1):63-70. doi: 10.1016/s1050-4648(02)00139-0.

DOI:10.1016/s1050-4648(02)00139-0

PMID:12787688

Abstract

A cDNA containing the gene for Japanese flounder IgD consisted of 3240 bp encoding 998 amino acid residues. The amino acid sequence of the constant region of Japanese flounder IgD shares 38-80% identity with the sequences of previously reported teleost IgDs. The structure of the constant region of Japanese flounder IgD, which contains the micro1, delta1, delta2, delta3, delta4, delta5, delta6, delta7, and TM regions, is similar to the structures of the constant regions of the IgDs of channel catfish and Atlantic salmon. Southern blot hybridisation showed that the Japanese flounder IgD gene exists as a single locus. The Japanese flounder IgD gene was mainly detected in peripheral blood leucocytes (PBLs) and small amounts were detected in the spleen, head and trunk kidney, although IgM mRNA was detected in similar amounts in PBLs, the head kidney, and spleen. The copy number of IgM mRNA in Japanese flounder PBL was 56-fold higher than that of IgD.

摘要

一个包含牙鲆免疫球蛋白D（IgD）基因的cDNA由3240个碱基对组成，编码998个氨基酸残基。牙鲆IgD恒定区的氨基酸序列与先前报道的硬骨鱼免疫球蛋白D序列具有38%-80%的同一性。牙鲆IgD恒定区的结构包含micro1、delta1、delta2、delta3、delta4、delta5、delta6、delta7和跨膜（TM）区域，与斑点叉尾鮰和大西洋鲑免疫球蛋白D恒定区的结构相似。Southern杂交显示牙鲆IgD基因以单一位点存在。牙鲆IgD基因主要在外周血白细胞（PBL）中检测到，在脾脏、头肾和躯干肾中检测到少量，尽管免疫球蛋白M（IgM）mRNA在PBL、头肾和脾脏中的检测量相似。牙鲆PBL中IgM mRNA的拷贝数比IgD高56倍。