Yang E V, Shima D T, Tassava R A
Department of Molecular Genetics, Ohio State University, Columbus 43210.
J Exp Zool. 1992 Dec 1;264(3):337-50. doi: 10.1002/jez.1402640312.
Monoclonal antibodies (mAb) utilized in regeneration studies to date identify antigens that are up-regulated in the blastema. We obtained a monoclonal antibody, designated ST1 (Stump 1), that is reactive to an extracellular matrix (ECM) antigen exhibiting the opposite distribution; ST1 is an abundant antigen of the limb stump soft tissues but is absent from within the blastema. The border between abundance and absence of mAb ST1 reactivity was sharp and extended as a concavity into the stump. This distinct dichotomy led to further studies relevant to understanding how this extracellular matrix antigen is modulated during regeneration. mAb ST1 reactivity decreased in the internal tissues at the distal end of the limb prior to blastema formation and remained absent until the onset of differentiation. The initial decrease in mAb ST1 reactivity was dependent on the combined effects of injury and the wound epithelium but was nerve independent. At blastema stages of regeneration, the distribution of tenascin, ascertained by mAb MT1 reactivity, closely matched the area without reactivity to mAb ST1. The spatial and temporal distribution of the ST1 antigen in unamputated limbs and during regeneration did not correspond to any previously described ECM component.
迄今为止，在再生研究中使用的单克隆抗体（mAb）识别的是在芽基中上调的抗原。我们获得了一种名为ST1（残端1）的单克隆抗体，它与一种表现出相反分布的细胞外基质（ECM）抗原发生反应；ST1是肢体残端软组织中的一种丰富抗原，但在芽基中不存在。mAb ST1反应性丰富与缺乏之间的边界很清晰，并以凹陷的形式延伸到残端。这种明显的二分法引发了进一步的研究，这些研究与理解这种细胞外基质抗原在再生过程中是如何被调节有关。在芽基形成之前，肢体远端内部组织中mAb ST1的反应性降低，并且在分化开始之前一直不存在。mAb ST1反应性的最初降低取决于损伤和伤口上皮的联合作用，但与神经无关。在再生的芽基阶段，通过mAb MT1反应性确定的肌腱蛋白的分布与对mAb ST1无反应的区域紧密匹配。ST1抗原在未截肢肢体和再生过程中的时空分布与任何先前描述的ECM成分均不对应。
