通过与支持细胞共移植和单次注射抗淋巴细胞血清对大鼠胰岛异种移植进行免疫保护。
Immunoprotection of rat islet xenografts by cotransplantation with sertoli cells and a single injection of antilymphocyte serum.
作者信息
Dufour Jannette M, Rajotte Ray V, Kin Tatsuya, Korbutt Gregory S
机构信息
Surgical-Medical Research Institute, University of Alberta, Edmonton, Canada.
出版信息
Transplantation. 2003 May 15;75(9):1594-6. doi: 10.1097/01.TP.0000058748.00707.88.
BACKGROUND
Islet transplantation has become a clinical reality; however, before it can be extended to young juvenile diabetics an unlimited supply of tissue is needed and the use of chronic immunosuppression should be eliminated. This study was designed to determine whether Sertoli cells can immunoprotect islet xenografts.
METHODS
Lewis rat islets were cotransplanted with Balb/c Sertoli cells in diabetic Balb/c mice treated with one injection of anti-mouse lymphocyte serum (ALS).
RESULTS
When islets were transplanted alone, in combination with Sertoli cells, or in combination with ALS, mean graft survival times were 10.9+/-0.8, 14.0+/-1.2, or 12.2+/-0.7, respectively. When islets were combined with Sertoli cells and ALS, mean graft survival time increased to 64.9+/-8.1.
CONCLUSIONS
Sertoli cells are able to prolong the survival of islet xenografts when combined with ALS, thereby supporting their use as a means to immunoprotect cellular grafts such as islets for the treatment of type 1 diabetes.
背景
胰岛移植已成为临床现实；然而，在将其推广至年轻的青少年糖尿病患者之前，需要有无限量的组织供应，并且应避免使用长期免疫抑制。本研究旨在确定支持细胞是否能够对胰岛异种移植物起到免疫保护作用。
方法
将Lewis大鼠胰岛与Balb/c支持细胞共同移植到经单次注射抗小鼠淋巴细胞血清（ALS）处理的糖尿病Balb/c小鼠体内。
结果
单独移植胰岛、与支持细胞联合移植或与ALS联合移植时，平均移植物存活时间分别为10.9±0.8、14.0±1.2或12.2±0.7。当胰岛与支持细胞及ALS联合移植时，平均移植物存活时间延长至64.9±8.1。
结论
支持细胞与ALS联合时能够延长胰岛异种移植物的存活时间，从而支持其作为免疫保护细胞移植物（如胰岛）用于治疗1型糖尿病的一种手段。
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