重离子照射后人成纤维细胞中染色质损伤的诱导及等臂染色单体断裂的分布

Induction of chromatin damage and distribution of isochromatid breaks in human fibroblast cells exposed to heavy ions.

作者信息

Kawata Tetsuya, Ito Hisao, Motoori Ken, Ueda Takuya, Shigematsu Naoyuki, Furusawa Yoshiya, Durante Marco, George Kerry, Wu Honglu, Cucinotta Francis A

机构信息

Department of Radiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

出版信息

J Radiat Res. 2002 Dec;43 Suppl:S169-73. doi: 10.1269/jrr.43.s169.

DOI:10.1269/jrr.43.s169

PMID:12793753

Abstract

The frequency of chromatid breaks and the distribution of isochromatid breaks were measured in G2-phase normal human fibroblasts prematurely condensed a short time after exposure to low- or high-LET radiations. The average number of isochromatid breaks from a single particle traversal increased with increasing LET values, while the average number of chromatid-type breaks appeared to reach a plateau. The distribution of isochromatid breaks after high-LET iron particles exposure was overdispersed compared to gamma-rays, indicating that a single iron particle traversal through a cell nucleus can produce multiple isochromatid breaks.

摘要

在G2期正常人成纤维细胞中，测量了暴露于低或高传能线密度（LET）辐射后短时间内过早浓缩的染色单体断裂频率和等染色单体断裂分布。单个粒子穿过产生的等染色单体断裂平均数量随LET值增加而增加，而染色单体型断裂的平均数量似乎达到了一个平台期。与γ射线相比，高LET铁粒子暴露后的等染色单体断裂分布过度分散，这表明单个铁粒子穿过细胞核可产生多个等染色单体断裂。