Lopez-Bote C J, Isabel B, Ruiz J, Daza A
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Arch Tierernahr. 2003 Feb;57(1):11-25. doi: 10.1080/0003942031000086653.
The experiment was organized in a 3 x 2 factorial arrangement with three dietary fat blends and a basal (20 mg kg(-1) diet) or supplemented (220 mg kg(-1)) level of alpha-tocopheryl acetate. Dietary vitamin E and monounsaturated to polyunsaturated fatty acid ratio (dietary MUFA/PUFA) affected muscle alpha-tocopherol concentration (alpha-tocopherol [log microg g(-1)] = 0.18 (+/- 0.105) + 0.0034 (+/- 0.0003) x dietary alpha-tocopherol [mg kg(-1) diet] (P < 0.0001) + 0.39 (+/- 0.122) x dietary MUFA/PUFA (P < 0.0036)). An interaction between dietary alpha-tocopherol and dietary MUFA/PUFA exists for microsome alpha-tocopherol concentration (alpha-tocopherol [log microg g(-1)] = 1.14 ( +/- 0.169) (P < 0.0001) + 0.0056 ( +/- 0.00099) x dietary alpha-tocopherol [mg kg(-1) diet] (P <0.0001) + 0.54 (+/- 0.206) x dietary MUFA/PUFA (P < 0.0131) - 0.0033 (+/- 0.0011) x dietary alpha-tocopherol [mg kg(-1))] x dietary MUFA/PUFA (P < 0.0067)), and hexanal concentration in meat (hexanal [ng x g(-1)] = 14807.9 (+/- 1489.8)- 28.8 (+/- 10.6) dietary alpha-tocopherol [mg x kg(-1)] (P < 0.01) - 8436.6 (+/- 1701.6) x dietary MUFA/PUFA (P < 0.001) + 24.0 (+/- 11.22) x dietary alpha-tocopherol-dietary MUFA/ PUFA (P < 0.0416)). It is concluded that partial substitution of dietary PUFA with MUFA lead to an increase in the concentration of alpha-tocopherol in muscle and microsome extracts. An interaction between dietary alpha-tocopherol and fatty acids exists, in which at low level of dietary vitamin E inclusion, a low MUFA/ PUFA ratio leads to a reduction in the concentration of alpha-tocopherol in microsome extracts and a concentration of hexanal in meat above the expected values.
该实验采用3×2析因设计，有三种膳食脂肪混合物以及α-生育酚醋酸酯的基础水平（20毫克/千克日粮）或添加水平（220毫克/千克）。膳食维生素E和单不饱和脂肪酸与多不饱和脂肪酸比例（膳食MUFA/PUFA）影响肌肉α-生育酚浓度（α-生育酚[对数微克/克]=0.18（±0.105）+0.0034（±0.0003）×膳食α-生育酚[毫克/千克日粮]（P<0.0001）+0.39（±0.122）×膳食MUFA/PUFA（P<0.0036））。膳食α-生育酚与膳食MUFA/PUFA之间存在交互作用，影响微粒体α-生育酚浓度（α-生育酚[对数微克/克]=1.14（±0.169）（P<0.0001）+0.0056（±0.00099）×膳食α-生育酚[毫克/千克日粮]（P<0.0001）+0.54（±0.206）×膳食MUFA/PUFA（P<0.0131） - 0.0033（±0.0011）×膳食α-生育酚[毫克/千克]×膳食MUFA/PUFA（P<0.0067）），以及肉中己醛浓度（己醛[纳克/克]=14807.9（±1489.8） - 28.8（±10.6）×膳食α-生育酚[毫克/千克]（P<0.01） - 8436.6（±1701.6）×膳食MUFA/PUFA（P<0.001）+24.0（±11.22）×膳食α-生育酚-膳食MUFA/PUFA（P<0.0416））。得出的结论是，用MUFA部分替代膳食PUFA会导致肌肉和微粒体提取物中α-生育酚浓度增加。膳食α-生育酚与脂肪酸之间存在交互作用，即在膳食维生素E添加水平较低时，低MUFA/PUFA比例会导致微粒体提取物中α-生育酚浓度降低，以及肉中己醛浓度高于预期值。
