Takahashi J B, Hoshimaru M, Jaye M, Kikuchi H, Hatanaka M
Department of Viral Oncology, Faculty of Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):398-405. doi: 10.1016/0006-291x(92)91572-8.
Acidic and basic fibroblast growth factors (aFGF and bFGF) are mitogens for mesoderm- and neuroectoderm-derived cells. The facts that FGF-related proteins are oncogenic and that FGFs are expressed in a variety of tumor cell lines or tumor tissues suggest the transforming activities of FGFs. To examine such an activity of aFGF, we introduced a human aFGF expression vector into two populations of Rat-1 cells; one was non-transformed (nRat-1), the other was partially-transformed (tRat-1). tRat-1 cells transfected with aFGF cDNA formed larger colonies in soft agar and produced larger and more malignant tumors in nude mice than those of parental cells. In contrast, nRat-1 cells transfected with aFGF cDNA neither formed colonies in soft agar nor produced tumors in nude mice. Our results suggest that high expression of aFGF may enhance a tumorigenic potential of Rat-1 cells rather than confer such a potential de novo.
酸性和碱性成纤维细胞生长因子（aFGF和bFGF）是中胚层和神经外胚层来源细胞的促分裂原。FGF相关蛋白具有致癌性，且FGFs在多种肿瘤细胞系或肿瘤组织中表达，这些事实提示FGFs具有转化活性。为了检测aFGF的这种活性，我们将人aFGF表达载体导入两群大鼠-1细胞中；一群是非转化细胞（nRat-1），另一群是部分转化细胞（tRat-1）。与亲本细胞相比，转染aFGF cDNA的tRat-1细胞在软琼脂中形成更大的集落，在裸鼠中产生更大且更具侵袭性的肿瘤。相反，转染aFGF cDNA的nRat-1细胞既不在软琼脂中形成集落，也不在裸鼠中产生肿瘤。我们的结果表明，aFGF的高表达可能增强大鼠-1细胞的致瘤潜能，而不是从头赋予这种潜能。
