卡氏肺孢子虫滋养体的纯化及其循环抗原的鉴定。

Ishimaru T, Shimono N, Sawae Y, Niho Y

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

J Clin Microbiol. 1992 Dec;30(12):3263-7. doi: 10.1128/jcm.30.12.3263-3267.1992.

We have developed a new method to isolate Pneumocystis carinii trophozoites from experimental rat bronchoalveolar lavage specimens by using a Percoll discontinuous gradient and have identified the circulating antigens in experimental rat P. carinii pneumonia. The antigenic components of the trophozoites were compared with those of cysts by immunoblotting. A major immunoreactive band of 90,000 Da and some others of lower molecular mass were found in trophozoites. On the other hand, bands of 110,000, 50,000, and 45,000 Da were observed in cysts. The band of 50,000 Da was not identified when antitrophozoite rabbit serum preabsorbed with P. carinii-infected rat serum was used for immunoblotting. These results suggest that the molecule of 50,000 Da is the major circulating antigen in P. carinii-infected rats.

我们开发了一种新方法，通过使用Percoll不连续梯度从实验大鼠支气管肺泡灌洗标本中分离卡氏肺孢子虫滋养体，并鉴定了实验大鼠卡氏肺孢子虫肺炎中的循环抗原。通过免疫印迹法比较了滋养体和包囊的抗原成分。在滋养体中发现了一条主要的90,000 Da免疫反应带和一些其他较低分子量的条带。另一方面，在包囊中观察到110,000、50,000和45,000 Da的条带。当用卡氏肺孢子虫感染大鼠血清预吸收的抗滋养体兔血清进行免疫印迹时，未鉴定出50,000 Da的条带。这些结果表明，50,000 Da的分子是卡氏肺孢子虫感染大鼠中的主要循环抗原。