暴发性肝炎的辅助性部分原位肝移植：猪模型中病变天然肝脏的再生
Auxiliary partial orthotopic liver transplantation for fulminant hepatitis: regeneration of the diseased native liver in a pig model.
作者信息
Ishiguro Shingo, Takada Yasutsugu, Gu Mei, Fukunaga Kiyoshi, Taniguchi Hdeki, Seino Kenichiro, Kawamoto Toru, Yuzawa Kenji, Otsuka Masaaki, Todoroki Takeshi, Fukao Katashi
机构信息
Graduate School of Medicine, University of Tsukuba, Tsukuba City, Ibaraki, Japan.
出版信息
Transplantation. 2003 Jun 15;75(11):1901-4. doi: 10.1097/01.TP.0000065293.75456.08.
BACKGROUND
This study was performed to develop a clinically relevant porcine model of auxiliary partial orthotopic liver transplantation (APOLT) for fulminant hepatic failure.
METHODS
FHF was induced by intraportal administration of alpha-amanitin and lipopolysaccharide. Thereafter, pigs were divided into four groups. Group 1 was an untreated, control group. In group 2, pigs underwent only a left hemihepatectomy. Pigs in groups 3 and 4 received APOLT after hemihepatectomy. Tacrolimus was administered to pigs in group 4, but not to those in group 3.
RESULTS
Two-week survival rates were 0%, 20%, 40%, and 100% for groups 1, 2, 3, and 4, respectively. In group 4, after abolishing the graft function at 1 week, pigs survived for more than 2 weeks, and regeneration of the native liver was confirmed histologically.
CONCLUSIONS
Pigs suffering from fulminant hepatic failure could achieve long survival and liver regeneration with a temporary support of the auxiliary graft.
背景
本研究旨在建立一种临床上适用的暴发性肝衰竭辅助性部分原位肝移植（APOLT）猪模型。
方法
通过门静脉注射鹅膏蕈碱和脂多糖诱导暴发性肝衰竭（FHF）。此后，将猪分为四组。第1组为未治疗的对照组。第2组猪仅接受左半肝切除术。第3组和第4组猪在半肝切除术后接受APOLT。第4组猪给予他克莫司，第3组猪未给予。
结果
第1、2、3和4组的两周生存率分别为0%、20%、40%和100%。在第4组中，在1周时废除移植物功能后，猪存活超过2周，并且组织学证实了原生肝脏的再生。
结论
患有暴发性肝衰竭的猪在辅助移植物的临时支持下可以实现长期存活和肝脏再生。
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