Singer-Sam J, LeBon J M, Dai A, Riggs A D
Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.
PCR Methods Appl. 1992 Feb;1(3):160-3. doi: 10.1101/gr.1.3.160.
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.
我们发现，单核苷酸引物延伸分析（一种目前用于定性测量DNA中等位基因差异的基于PCR的分析方法）可用于定量测量仅相差一个核苷酸的等位基因特异性转录本。我们表明，即使在含有Pgk-1b转录本的RNA过量1000倍的情况下，也能特异性检测到含有Pgk-1a转录本的总RNA。
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