维生素D受体基因多态性与肺结核易感性的病例对照研究

[A case-control study on the vitamin D receptor gene polymorphisms and susceptibility to pulmonary tuberculosis].

作者信息

Liu Wei, Zhang Cui-ying, Wu Xiao-ming, Tian Lei, Li Chun-zhi, Zhao Qiu-min, Zhang Pan-he, Yang Shu-mei, Yang Hong, Zhang Xi-tan, Cao Wu-chun

机构信息

Epidemiological Department of Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2003 May;24(5):389-92.

PMID:12820934

Abstract

OBJECTIVE

To investigate the association between the genetic polymorphisms of VDR gene and susceptibility to pulmonary tuberculosis.

METHODS

Case-control study was conducted. PCR-RFLP technique was used to detect the C/T polymorphism in VDR gene. Information on related factors of tuberculosis was collected using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.

RESULTS

A sample of 76 cases and 171 controls was studied. The genotype frequencies of VDR-FF, VDR-Ff and VDR-ff were 38.2%, 44.7%, 17.1% and 52.6%, 40.9%, 6.4% respectively. VDR-ff was significantly overrepresented in case group, the OR (95% CI) was 3.668 (1.483 - 9.071) when comparing with FF genotype. The significant association remained after adjusting BCG immunization and smoking, the OR (95% CI) was 3.036 (1.117 - 8.253).

CONCLUSION

The VDR-ff genotype might be associated with the susceptibility to pulmonary tuberculosis in Chinese Han population.

摘要

目的

探讨维生素D受体（VDR）基因多态性与肺结核易感性之间的关联。

方法

进行病例对照研究。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测VDR基因的C/T多态性。使用预先测试的问卷收集结核病相关因素的信息。采用SPSS软件包进行单因素和多因素逻辑分析。

结果

研究了76例病例和171例对照。VDR-FF、VDR-Ff和VDR-ff基因型频率分别为38.2%、44.7%、17.1%和52.6%、40.9%、6.4%。VDR-ff在病例组中显著过多，与FF基因型相比，比值比（OR，95%可信区间）为3.668（1.483 - 9.071）。在调整卡介苗免疫和吸烟因素后，显著关联仍然存在，OR（95%可信区间）为3.036（1.117 - 8.253）。