在基质辅助激光解吸电离质谱（MALDI-MS）分析过程中，通过4-二甲基氨基苯基偶氮苯基-4'-马来酰亚胺衍生物的快速碎片化来识别含半胱氨酸的肽段。
Recognition of cysteine-containing peptides through prompt fragmentation of the 4-dimethylaminophenylazophenyl-4'-maleimide derivative during analysis by MALDI-MS.
作者信息
Borges Chad R, Watson J Throck
机构信息
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.
出版信息
Protein Sci. 2003 Jul;12(7):1567-72. doi: 10.1110/ps.0301403.
Abstract
Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4'-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.
摘要
在特定的紫外基质辅助激光解吸电离（UV-MALDI）条件下，含半胱氨酸肽段的4-二甲基氨基苯基偶氮苯基-4'-马来酰亚胺（DABMI）衍生物会迅速裂解，产生特征性的质谱图。正如其他人之前所报道的，用发色团DABMI进行衍生化，可使含半胱氨酸的肽段和蛋白质在高效液相色谱（HPLC）过程中通过在紫外和可见光的较高波长处的吸光度进行追踪。在此，我们描述了一种方法，通过该方法这些相同的肽衍生物可凭借其独特的MALDI质谱裂解模式被识别，然后进行质量定位，从而便于快速鉴定含半胱氨酸的肽段。
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