Zhang Hui, Yu Chen, Liu Gang-yi, Jia Jing-ying, Hong You-cai, Xu Xiu-rong
Shanghai Xu Hui District Central Hospital, Shanghai 200031, China.
Yao Xue Xue Bao. 2003 Mar;38(3):215-7.
To establish an HPLC-fluorescent spectrometric method for the determination of mexiletine hydrochloride in plasma after derivatization with fluram.
Fluram acetone solution was added to the deproteinized plasma with acetone to obtain the derivative of mexiletine. The HPLC method was performed on a column of Allitima C18 (150 mm x 4.6 mm, 5 microns) with the mobile phase of methanol-water-diethylamine-phosphoric acid buffer (2.4 mol.L-1, pH 4.0) (70:28:2), and the detective wavelength were set at Ex 392 nm and Em 480 nm.
Mexiletine has a liner range over the concentration range from 0.100-6.400 mg.L-1. The lowest detectable concentration of this method was 5 micrograms.L-1 (S/N > or = 4). The intra-day and inter-day RSDs were 1.34%-5.31%, respectively.
This method is simple, selective and can be used for therapeutic drug monitoring (TDM) and pharmacokinetic studies of mexiletine.
建立一种用氟胺衍生化后以高效液相色谱 - 荧光光谱法测定血浆中盐酸美西律的方法。
向用丙酮脱蛋白的血浆中加入氟胺丙酮溶液以获得美西律的衍生物。采用高效液相色谱法，色谱柱为Allitima C18（150 mm×4.6 mm，5微米），流动相为甲醇 - 水 - 二乙胺 - 磷酸缓冲液（2.4 mol·L⁻¹，pH 4.0）（70:28:2），检测波长设定为激发波长392 nm和发射波长480 nm。
美西律在0.100 - 6.400 mg·L⁻¹浓度范围内呈线性关系。该方法的最低检测浓度为5微克·L⁻¹（信噪比≥4）。日内和日间相对标准偏差分别为1.34% - 5.31%。
该方法简便、具有选择性，可用于美西律的治疗药物监测（TDM）和药代动力学研究。
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