Launois P, N'Diaye M, Sarthou J L, Millan J, Bach M A
Immunologie Cellulaire, Institut Pasteur de Dakar, Senegal.
J Autoimmun. 1992 Dec;5(6):745-57. doi: 10.1016/0896-8411(92)90190-2.
Binding of leprosy sera to peripheral nerve from different species (mouse, guinea pig and rabbit) was evaluated by ELISA. A majority of sera, whatever the clinical form of leprosy, bind to these antigens. Absorption with Mycobacterium bovis BCG demonstrated that these antibodies recognize cross-reactive epitopes between peripheral nerve and mycobacteria. In immunoblot analysis, both leprosy patient sera and a monoclonal antibody directed at the 65 kDa heat shock protein of M. leprae were shown to react with a heat-shock 67-68 kDa sciatic nerve protein. Binding of the monoclonal antibody to this sciatic nerve antigen was prevented by incubation with lepromatous patient sera, showing that some peripheral nerve epitopes recognized by patient antibodies are shared by the 65 kDa heat shock protein of M. leprae.
通过酶联免疫吸附测定（ELISA）评估麻风血清与不同物种（小鼠、豚鼠和兔子）外周神经的结合情况。无论麻风的临床类型如何，大多数血清都能与这些抗原结合。用卡介苗（Mycobacterium bovis BCG）吸收实验表明，这些抗体识别外周神经和分枝杆菌之间的交叉反应表位。在免疫印迹分析中，麻风患者血清和一种针对麻风杆菌65 kDa热休克蛋白的单克隆抗体均显示与坐骨神经的一种67 - 68 kDa热休克蛋白发生反应。用瘤型麻风患者血清孵育可阻止单克隆抗体与这种坐骨神经抗原的结合，表明患者抗体识别的一些外周神经表位与麻风杆菌的65 kDa热休克蛋白共有。
