间接法ELISA检测牛血清中抗牛腹泻病毒抗体
[Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum].
作者信息
Pacheco J M, Lager I
机构信息
Instituto de Virología, CICV-INTA, Castelar, CC. 77, 1708 Morón, Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 2003 Jan-Mar;35(1):19-23.
Serological diagnosis is very important for the detection of bovine viral diarrhea virus, an important pathogen related to reproductive failure. Methods normally used for the detection of antibodies are serum neutralization (SN) and ELISA. The objective of this work was to standardize an indirect ELISA with SN. With a diagnostic sensitivity of 98.31% and a diagnostic specificity of 100%, this ELISA-BVDV shows good sensitivity, specificity and repeatability. It is easy to transfer, economical, and easy to perform.
血清学诊断对于检测牛病毒性腹泻病毒非常重要，该病毒是一种与繁殖障碍相关的重要病原体。通常用于检测抗体的方法是血清中和试验（SN）和酶联免疫吸附测定（ELISA）。这项工作的目的是标准化一种结合血清中和试验的间接酶联免疫吸附测定。这种牛病毒性腹泻病毒酶联免疫吸附测定（ELISA-BVDV）的诊断敏感性为98.31%，诊断特异性为100%，具有良好的敏感性、特异性和重复性。它易于推广、经济且操作简便。
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